Lynn R K, Leger R M, Gordon W P, Olsen G D, Gerber N
J Chromatogr. 1977 Jan 21;131:329-40. doi: 10.1016/s0021-9673(00)80949-8.
A new gas chromatographic assay utilizing 2-dimethylamino-4,4-diphenyl-5-nonanone as the internal standard was developed for the quantification of methadone. The method involved extraction of methadone with 1-chlorobutane from tissue at pH 9.8, re-extraction of an aliquot of the organic solvent with 0.5 M sulphuric acid, alkalinization and final extraction into chloroform. The assay was used to determine the concentration of methadone (i) in whole blood samples from a normal volunteer following a single 9.4-mg oral dose of d-methadone hydrochloride, (ii) in whole blood, saliva and gastric juice from a methadone addict maintained on 90 mg of dimethadone hydrochloride per day, (iii) in mouse liver microsomes incubated with methadone, and (iv) in the perfusate of the isolated perfused rat liver.
建立了一种新的气相色谱测定法，以2-二甲基氨基-4,4-二苯基-5-壬酮作为内标物来定量美沙酮。该方法包括在pH 9.8条件下用1-氯丁烷从组织中提取美沙酮，用0.5 M硫酸对一份有机溶剂进行再提取，碱化并最终萃取到氯仿中。该测定法用于确定以下样品中美沙酮的浓度：(i) 一名正常志愿者单次口服9.4 mg盐酸d-美沙酮后全血样品中的美沙酮浓度；(ii) 一名每天服用90 mg盐酸二美沙酮维持治疗的美沙酮成瘾者的全血、唾液和胃液中的美沙酮浓度；(iii) 与美沙酮一起孵育的小鼠肝微粒体中的美沙酮浓度；以及(iv) 离体灌注大鼠肝脏的灌注液中的美沙酮浓度。
