Miyazaki H, Ishibashi M, Itoh M
J Chromatogr. 1977 May 11;135(1):109-16. doi: 10.1016/s0021-9673(00)86306-2.
In order to apply the intensity-matching technique to polyhydroxysteroids in biological fluids, the doublet of [M]+ and [M+3]+ was prepared in the process of derivatization by use of a mixture of trimethylsilylimidazole (TSIM) and TSIM-d3 and was used for mass fragmentography. The molar ratio of TSIM and TSIM-d3 was defined by the reciprocal of the number of hydroxyl groups in steroids. This technique may be useful in identifying low levels of the endogenous polyhydroxysteroids of interest in biological fluids.
为了将强度匹配技术应用于生物流体中的多羟基甾体，在衍生化过程中，通过使用三甲基硅咪唑（TSIM）和TSIM-d3的混合物制备了[M]+和[M+3]+的双峰，并将其用于质量碎片分析法。TSIM和TSIM-d3的摩尔比由甾体中羟基数量的倒数确定。该技术可能有助于鉴定生物流体中低水平的内源性目标多羟基甾体。
