Kerdelhué B, Jones G S, Gordon K, Seltman H, Lenoir V, Mélik Parsadaniantz S, Williams R F, Hodgen G D
Laboratoire de Neurobiologie de la Reproduction, CNRS, Jouy-en-Josas, France.
J Neurosci Res. 1995 Oct 1;42(2):228-35. doi: 10.1002/jnr.490420210.
The present work describes time-dependent changes in the content of corticotropin-releasing hormone (CRH), adrenocorticotropin (ACTH), and beta-endorphin (beta-EP) in the hypothalamus (HT) and anterior pituitary (AP) and in the concentration of ACTH and beta-EP in the plasma during the 17 beta estradiol (E2) benzoate (E2B)-induced luteinizing hormone (LH) surge in ovariectomized cynomolgus monkeys. Monkeys were euthanized at 0, 30, 48, 72, and 96 hr post-E2B. HT and AP were rapidly dissected, extracted in 2 N acetic acid containing 1 mM phenylmethane sulfonyl fluoride at 4 degrees C, and centrifuged at 18,000g for 30 min. Peptide concentrations were measured in the supernatant by specific radioimmunoassays (RIAs). In the HT, there were significant (P < 0.05) decreases in ACTH and beta-EP content by 30 hr post-E2B and a significant (P < 0.05) decrease in HT CRH content 48 hr post-E2B. Thereafter, CRH, ACTH, and beta-EP content increased up to 72 hr post-E2B. In the AP, there was an almost linear decrease in the CRH content through 48 hr post-E2B followed by a marked 20-fold (P < 0.01) increase in the AP CRH content at 72 hr post-E2B, which corresponds to the time of the descending arm of the LH surge. The patterns of ACTH and beta-EP content were very similar in the AP, while that of CRH differed markedly. In contrast, in the HT CRH, ACTH, and beta-EP profiles were very similar. Significant (P < 0.05) increases in circulating levels of ACTH, beta-EP, and cortisol were evident at 30 hr (all 3 hormones), 48 hr (beta-EP and cortisol), and 72 hr (cortisol) post-E2B, which corresponds with the time of decreased hypothalamic content of CRH, ACTH, and beta-EP. These results suggest that there maybe a marked activation of the hypothalamo-anterior pituitary-adrenal axis during the negative and positive feedback phases of the E2B-induced LH surge in the ovariectomized monkey.
本研究描述了在去卵巢食蟹猴中，17β-雌二醇（E2）苯甲酸酯（E2B）诱导促黄体生成素（LH）激增期间，下丘脑（HT）和垂体前叶（AP）中促肾上腺皮质激素释放激素（CRH）、促肾上腺皮质激素（ACTH）和β-内啡肽（β-EP）含量的时间依赖性变化，以及血浆中ACTH和β-EP浓度的变化。在给予E2B后0、30、48、72和96小时对猴子实施安乐死。迅速解剖HT和AP，在含有1 mM苯甲基磺酰氟的2 N乙酸中于4℃提取，然后在18,000g下离心30分钟。通过特异性放射免疫测定法（RIA）测量上清液中的肽浓度。在HT中，给予E2B后30小时ACTH和β-EP含量显著（P < 0.05）下降，给予E2B后48小时HT CRH含量显著（P < 0.05）下降。此后，CRH、ACTH和β-EP含量在给予E2B后72小时内增加。在AP中，直到给予E2B后48小时CRH含量几乎呈线性下降，随后在给予E2B后72小时AP CRH含量显著增加20倍（P < 0.01），这与LH激增的下降臂时间相对应。AP中ACTH和β-EP含量的模式非常相似，而CRH的模式明显不同。相比之下，HT中CRH、ACTH和β-EP的概况非常相似。给予E2B后30小时（所有3种激素）、48小时（β-EP和皮质醇）和72小时（皮质醇）时，循环中ACTH水平、β-EP水平和皮质醇水平显著（P < 0.05）升高，这与下丘脑CRH、ACTH和β-EP含量降低的时间相对应。这些结果表明，在去卵巢猴子中，E2B诱导的LH激增的负反馈和正反馈阶段，下丘脑-垂体前叶-肾上腺轴可能有明显激活。
