Kloots W J, van Amelsvoort J M, Brink E J, Ritskes J, Remie R
Unilever Research Laboratorium, Vlaardingen, The Netherlands.
Lab Anim Sci. 1995 Oct;45(5):588-91.
A method was developed to surgically implant a silicone/stainless steel fistula-cannula in the cecum of freely moving rats. The specially designed fistula-cannula allows sampling of cecal contents at any time without disturbing the physiologic functions of the intestinal tract, as evaluated by observation of general well-being and postmortem macroscopic inspection. The fistula-cannula was implanted in 12 male rats under general anesthesia. The animals remained in good health during the postoperative period lasting up to 9 weeks. They gained weight normally compared with a control group (n = 8) that had not undergone surgery. Samples of 0.2 to 1.0 g of contents could be collected with a microspatula, without the need to anesthetize the animal. The model provides a useful system for sampling cecal contents without the need to sacrifice the animal. Because it allows cross-over studies to be carried out, this approach may result in significantly reducing the number of animals required for digestive studies.
开发了一种手术方法，用于在自由活动的大鼠盲肠中植入硅胶/不锈钢瘘管插管。通过观察大鼠的整体健康状况和死后大体检查评估，这种专门设计的瘘管插管能够在不干扰肠道生理功能的情况下随时采集盲肠内容物。在全身麻醉下，将瘘管插管植入12只雄性大鼠体内。在长达9周的术后期间，这些动物保持健康。与未接受手术的对照组（n = 8）相比，它们体重正常增加。使用微型刮铲可以采集0.2至1.0克内容物样本，无需对动物进行麻醉。该模型提供了一个有用的系统，无需牺牲动物即可采集盲肠内容物。由于它允许进行交叉研究，这种方法可能会显著减少消化研究所需的动物数量。
