Mantha S V, Kalra J, Prasad K
Department of Physiology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
Life Sci. 1996;58(6):503-9. doi: 10.1016/0024-3205(95)02315-1.
Effects of high cholesterol diet (0.5% and 1%) on the activity of antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px)] in the aortic tissue of rabbits were investigated in the absence or presence of probucol (0.5 gm/kg daily, orally). Five groups of ten rabbits each were studied. Group I, regular rabbit chow diet; Group II, chow + 0.5% cholesterol; Group III, chow + 0.5% cholesterol+probucol; Group IV, chow + 1% cholesterol and Group V, chow + 1% cholesterol+probucol. The aorta was removed at the end of 4 months for measurement of the antioxidant enzymes. An increase in activity of aortic antioxidant enzymes was noted in cholesterol-fed rabbits (Groups II and IV), being similar for SOD and catalase but higher for GSH-Px in Group IV as compared to Group II. Probucol was ineffective in altering this cholesterol-induced increase in enzyme activity except in Group III where it increased the activity of GSH-Px. These results suggest that aortic antioxidant enzymes are affected in hypercholesterolemia and that probucol is ineffective in altering the aortic antioxidant enzyme activity except GSH-Px activity which increased in 0.5% cholesterol-fed rabbits. The protective effects of probucol against hypercholesterolemic atherosclerosis may be partly due to an increase in the GSH-Px activity at low levels of hypercholesterolemia. At higher levels of hypercholesterolemia, the protective effects of probucol could be due to its antioxidant activity.
研究了高胆固醇饮食（0.5%和1%）对兔主动脉组织中抗氧化酶[超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）]活性的影响，实验分别在给予或未给予普罗布考（每日0.5克/千克，口服）的情况下进行。研究了五组兔子，每组十只。第一组，常规兔粮饮食；第二组，兔粮+0.5%胆固醇；第三组，兔粮+0.5%胆固醇+普罗布考；第四组，兔粮+1%胆固醇；第五组，兔粮+1%胆固醇+普罗布考。4个月结束时取出主动脉，测量抗氧化酶活性。喂食胆固醇的兔子（第二组和第四组）主动脉抗氧化酶活性增加，SOD和CAT活性相似，但第四组的GSH-Px活性高于第二组。普罗布考对这种胆固醇诱导的酶活性增加无效，第三组除外，在该组中普罗布考增加了GSH-Px的活性。这些结果表明，高胆固醇血症会影响主动脉抗氧化酶，普罗布考对改变主动脉抗氧化酶活性无效，但在喂食0.5%胆固醇的兔子中，普罗布考增加了GSH-Px的活性。普罗布考对高胆固醇血症性动脉粥样硬化的保护作用可能部分归因于在轻度高胆固醇血症时GSH-Px活性的增加。在较高水平的高胆固醇血症时，普罗布考的保护作用可能归因于其抗氧化活性。
