Rózalska B, Ljungh A, Burow A, Rudnicka W
Department of Infectious Biology, University of Lódź, Poland.
Microbiol Immunol. 1995;39(7):443-50. doi: 10.1111/j.1348-0421.1995.tb02227.x.
Candida yeasts are frequently isolated from patients with continuous ambulatory peritoneal dialysis peritonitis or other biomaterial-associated infections. The mouse model of candidal peritonitis was used to study the interaction of Candida cells with end-point attached heparinized polyethylene (H-PE) and with polymorphonuclear leukocytes (PMNs) or macrophages (M phi). Two Candida strains differing in cell surface hydrophobicity and in expression of fibronectin (Fn) binding were used for the study. Cells of both Candida strains adhered at higher numbers to H-PE surfaces preadsorbed with Fn or with human dialysis fluid (HDF) than to non-modified H-PE, supporting a role of Fn in mediating adhesion. C. albicans 4016 cells expressing low hydrophobicity and low binding of soluble Fn demonstrated stronger adhesion to PMNs than the more hydrophobic C. albicans 3248 yeasts, which express high binding of soluble Fn. However, C. albicans 4016 cells were more resistant to phagocytic killing and were hardly eradicated in intraperitoneally infected mice. The animals depleted in PMNs by treatment with CY were neither able to eradicate C. albicans 3248 (rapidly eliminated by normal mice) nor C. albicans 4016 yeasts (with a tendency to persist in the tissues of normal mice).
念珠菌酵母经常从持续性非卧床腹膜透析腹膜炎患者或其他生物材料相关感染患者中分离出来。念珠菌性腹膜炎的小鼠模型用于研究念珠菌细胞与终点附着的肝素化聚乙烯（H-PE）以及与多形核白细胞（PMN）或巨噬细胞（M phi）之间的相互作用。研究使用了两种在细胞表面疏水性和纤连蛋白（Fn）结合表达方面存在差异的念珠菌菌株。与未修饰的H-PE相比，两种念珠菌菌株的细胞对预先吸附有Fn或人透析液（HDF）的H-PE表面的粘附数量更多，这支持了Fn在介导粘附中的作用。表达低疏水性和低可溶性Fn结合的白色念珠菌4016细胞比表达高可溶性Fn结合的疏水性更强的白色念珠菌3248酵母对PMN的粘附更强。然而，白色念珠菌4016细胞对吞噬杀伤更具抗性，并且在腹腔感染的小鼠中很难被根除。用环磷酰胺（CY）处理使PMN耗竭的动物既无法根除白色念珠菌3248（正常小鼠能迅速清除），也无法根除白色念珠菌4016酵母（在正常小鼠组织中有持续存在的趋势）。
