Boeve C M, Wyngaerd A V, De Ley M
Laboratory for Biochemistry, Katholieke Universiteit Leuven, Heverlee, Belgium.
Mol Pharmacol. 1996 Jan;49(1):58-62.
We applied antisense RNA technology for reducing the level of human IFN-gamma (HuIFN-gamma) expression. An antisense RNA vector containing the full-length HuIFN-gamma cDNA in the opposite orientation was electroporated into cells constitutively producing very high levels of the cytokine. Approximately 53% of the resulting clones exhibited a specific HuIFN-gamma inhibition of an average of 95.5%. The results of reverse transcription-polymerase chain reaction and Northern blot analyses revealed that the antisense effect originated from a specific reduction of the targeted mRNA caused by antisense RNA expression. This very effective antisense RNA strategy can have possible therapeutic applications in treating diseases where HuIFN-gamma is known to play a negative role, such as in certain autoimmune diseases.
我们应用反义RNA技术来降低人类γ干扰素（HuIFN-γ）的表达水平。将一个含有全长HuIFN-γ cDNA且方向相反的反义RNA载体通过电穿孔导入持续产生高水平该细胞因子的细胞中。大约53%的所得克隆表现出对HuIFN-γ的特异性抑制，平均抑制率为95.5%。逆转录-聚合酶链反应和Northern印迹分析结果表明，反义效应源于反义RNA表达导致的靶向mRNA的特异性减少。这种非常有效的反义RNA策略在治疗已知HuIFN-γ起负面作用的疾病（如某些自身免疫性疾病）中可能具有治疗应用。
