一种用于区分结核分枝杆菌与结核分枝杆菌复合群的多重聚合酶链反应。

A multiplex polymerase chain reaction for distinguishing Mycobacterium tuberculosis from Mycobacterium tuberculosis complex.

作者信息

Sinclair K, Challans J A, Kazwala R R, Hewinson R G, Sharp J M

机构信息

Moredun Research Institute, Edinburgh, Scotland, UK.

出版信息

Mol Cell Probes. 1995 Oct;9(5):291-5. doi: 10.1016/s0890-8508(95)91524-9.

DOI:10.1016/s0890-8508(95)91524-9

PMID:8569767

Abstract

A multiplex polymerase chain reaction has been developed which is able to distinguish Mycobacterium tuberculosis from other members of the M. tuberculosis complex. The assay is based on the simultaneous amplification of two different targets: a 396bp region from the mtp40 species-specific gene sequence of M. tuberculosis and a 245bp fragment from the M. tuberculosis complex insertion sequence IS986. Results have been obtained for 54 mycobacterial strains including five non-M. tuberculosis complex isolates. All 49 strains of the M. tuberculosis complex were positive for IS986 but only the 27 M. tuberculosis isolates were positive for both IS986 and mtp40.

摘要

已开发出一种多重聚合酶链反应，它能够区分结核分枝杆菌与结核分枝杆菌复合群的其他成员。该检测方法基于同时扩增两个不同的靶标：来自结核分枝杆菌mtp40种特异性基因序列的396bp区域和来自结核分枝杆菌复合群插入序列IS986的245bp片段。已对54株分枝杆菌菌株进行了检测，其中包括5株非结核分枝杆菌复合群分离株。所有49株结核分枝杆菌复合群菌株的IS986检测均为阳性，但只有27株结核分枝杆菌分离株的IS986和mtp40检测均为阳性。