Eicher E M, Shown E P, Washburn L L
Jackson Laboratory, Bar Harbor, Maine 04609, USA.
Philos Trans R Soc Lond B Biol Sci. 1995 Nov 29;350(1333):263-8; discussion 268-9. doi: 10.1098/rstb.1995.0160.
C57BL/6J mice carrying a Mus domesticus poschiavinus Y chromosome (YPOS) develop as females with ovarian tissue or as hermaphrodites with ovarian and testicular tissue. We tested the hypothesis that the Y-linked component of this inherited sex reversal is caused by the M. d. poschiavinus Y-linked testis determining gene (symbolized Tdy or Sry) by examining gonadal development in C57BL/6J XYPOS mice carrying a M. musculus allele of Sry as a transgene. We found that in the presence of the transgene, XYPOS mice developed exclusively testicular tissue. This result indicates that the Sry allele carried on the YPOS chromosome is responsible for development of ovarian tissue in the C57BL/6J inbred strain background. We discuss this finding in light of DNA polymorphisms present in Sry alleles carried by various M. domesticus and M. musculus Y chromosomes. In addition, we present a hypothesis concerning the timing of expression of the testicular and ovarian determining genes in the developing fetal gonad based on the organization of ovarian and testicular tissue in ovotestes.
携带小家鼠波氏亚种Y染色体（YPOS）的C57BL/6J小鼠发育为具有卵巢组织的雌性，或发育为具有卵巢和睾丸组织的雌雄同体。我们通过检测携带小家鼠Sry等位基因作为转基因的C57BL/6J XYPOS小鼠的性腺发育情况，来验证这一遗传性性别反转的Y连锁成分是由小家鼠波氏亚种Y连锁的睾丸决定基因（符号为Tdy或Sry）引起的这一假设。我们发现，在存在转基因的情况下，XYPOS小鼠仅发育出睾丸组织。这一结果表明，YPOS染色体上携带的Sry等位基因是导致C57BL/6J近交系背景下卵巢组织发育的原因。我们根据各种小家鼠和小家鼠Y染色体携带的Sry等位基因中存在的DNA多态性来讨论这一发现。此外，我们基于卵睾中卵巢和睾丸组织的组织学结构，提出了一个关于发育中的胎儿性腺中睾丸和卵巢决定基因表达时间的假设。
