Anselmino C, Averbeck D, Cadet J
CEA/Département de Recherche Fondamentale sur la Matière Condensée, SESAM/LAN, Grenoble, France.
Photochem Photobiol. 1995 Dec;62(6):997-1004. doi: 10.1111/j.1751-1097.1995.tb02399.x.
The photoreaction of the furan-side moiety of 5-methoxypsoralen (5-MOP) with thymidine used as a DNA model compound was investigated in the dry state. Under these conditions, two main fluorescent photoadducts were formed and isolated by HPLC. The two modified nucleosides were characterized as the two cis-syn diastereoisomers of furan-side monoadducts of 5-MOP to thymidine on the basis of spectroscopic measurements including UV, fluorescence, 1H-NMR and circular dichroism analysis. The identification and quantification of the latter photoproducts within naked DNA exposed to photoexcited 5-MOP were achieved by enzymatic digestion completed by HPLC separation and fluorescence detection. Similarly, the two cis-syn furan-side monoadducts were found to be formed in the DNA of Saccharomyces cerevisiae cells after incubation with 5-MOP and subsequent exposure to 365 nm at an incident dose of 38.4 kJ m-2. Under these conditions, the rate of induction of two diastereoisomeric photoadducts was as low as one modification per 10(6) and 2 x 10(5) bases, respectively.
在干燥状态下，研究了5-甲氧基补骨脂素（5-MOP）呋喃侧部分与用作DNA模型化合物的胸腺嘧啶核苷的光反应。在这些条件下，通过高效液相色谱法（HPLC）形成并分离出两种主要的荧光光加合物。基于包括紫外、荧光、1H-核磁共振和圆二色性分析在内的光谱测量，这两种修饰的核苷被表征为5-MOP与胸腺嘧啶核苷呋喃侧单加合物的两种顺式-顺式非对映异构体。通过HPLC分离和荧光检测完成的酶促消化，实现了在暴露于光激发的5-MOP的裸露DNA中对后一种光产物的鉴定和定量。同样，在酿酒酵母细胞的DNA中，5-MOP孵育并随后以38.4 kJ m-2的入射剂量暴露于365 nm后，发现形成了两种顺式-顺式呋喃侧单加合物。在这些条件下，两种非对映异构光加合物的诱导率分别低至每10(6)和2×10(5)个碱基一次修饰。
