Amano M, Mukai H, Ono Y, Chihara K, Matsui T, Hamajima Y, Okawa K, Iwamatsu A, Kaibuchi K
Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma, Japan.
Science. 1996 Feb 2;271(5249):648-50. doi: 10.1126/science.271.5249.648.
Rho, a Ras-like small guanosine triphosphatase, has been implicated in cytoskeletal responses to extracellular signals such as lysophosphatidic acid (LPA) to form stress fibers and focal contacts. The form of RhoA bound to guanosine triphosphate directly bound to and activated a serine-threonine kinase, protein kinase N (PKN). Activated RhoA formed a complex with PKN and activated it in COS-7 cells. PKN was phosphorylated in Swiss 3T3 cells stimulated with LPA, and this phosphorylation was blocked by treatment of cells with botulinum C3 exoenzyme. Activation of Rho may be linked directly to a serine-threonine kinase pathway.
Rho是一种类Ras小GTP酶，它参与细胞骨架对细胞外信号（如溶血磷脂酸，LPA）的反应，以形成应力纤维和粘着斑。与三磷酸鸟苷结合的RhoA形式直接结合并激活了一种丝氨酸 - 苏氨酸激酶，即蛋白激酶N（PKN）。活化的RhoA在COS - 7细胞中与PKN形成复合物并激活它。在用LPA刺激的瑞士3T3细胞中PKN发生磷酸化，而这种磷酸化可通过用肉毒杆菌C3外毒素处理细胞来阻断。Rho的激活可能直接与丝氨酸 - 苏氨酸激酶途径相关。
