Gandolfi A J, Brendel K, Fisher R L, Michaud J P
Department of Pharmacology, University of Arizona, Tucson 85724, USA.
Toxicology. 1995 Dec 28;105(2-3):285-90. doi: 10.1016/0300-483x(95)03224-4.
Precision-cut tissue slices have proven to be a useful in vitro system for biotransformation and toxicity studies. Since tissue slices can be readily prepared from a variety of tissues and species, they can easily be used for interspecies investigations and comparisons. Furthermore, slices can be readily prepared from human tissue, thus comparisons (extrapolation) can be made between laboratory animals and humans. Slices can also be used to examine the toxic interactions of chemicals in vitro. It is important to use the correct experimental design to demonstrate toxic interactions and to assure that the tissue slices are properly exposed to the chemicals. Overall, tissue slices offer a valid in vitro system for performing species comparisons and chemical-chemical interaction studies.
精密切割组织切片已被证明是用于生物转化和毒性研究的一种有用的体外系统。由于组织切片可以很容易地从各种组织和物种中制备，它们可以很容易地用于种间研究和比较。此外，切片可以很容易地从人体组织制备，因此可以在实验动物和人类之间进行比较（外推）。切片还可用于体外检测化学物质的毒性相互作用。使用正确的实验设计来证明毒性相互作用并确保组织切片正确暴露于化学物质是很重要的。总体而言，组织切片为进行物种比较和化学物质相互作用研究提供了一个有效的体外系统。
