Quicke D L, Brace R C
J Microsc. 1979 Mar;115(2):161-3. doi: 10.1111/j.1365-2818.1979.tb00165.x.
A staining procedure is described with differentiates with distinct colours, cobalt and nickel ions introduced into neurones. Densely coloured precipitates are produced with these ions when rubeanic acid, a spot-test reagent, is added. Formation of the coloured complexes takes place within a few minutes, and may be followed under the dissecting microscope. This technique permits observation of the relative positions, within ganglia, of somata related to two emergent nerves following multiple axonal-backfilling. It also appears to be of value in tracing neurone branching after the intracellular injection of ions.
本文描述了一种染色方法，该方法通过将钴离子和镍离子引入神经元，使其呈现出不同的颜色。当加入斑点试验试剂硫氰酸时，这些离子会产生颜色浓郁的沉淀。在几分钟内就会形成有色络合物，并且可以在解剖显微镜下进行观察。该技术允许观察在多次轴突回灌后，神经节内与两条传出神经相关的胞体的相对位置。在细胞内注入离子后追踪神经元分支方面，该技术似乎也具有一定价值。
