Rafique M, Adachi W, Shiohara E
Department of Surgery, Shinshu University School of Medicine, Nagano-ken, Japan.
Anticancer Res. 1995 Sep-Oct;15(5B):1869-72.
The results of adjuvant continuous intraportal chemotherapy in preventing hepatic metastasis, following curative colorectal surgery, are still equivocal. To improve the efficacy of this treatment we tried to establish an experimental model for continuous intraportal infusion in Wistar male rats. Using a drug infusion balloon catheter, which can be fixed on the back of the rat, the continuous intraportal administration of chemo/immunotherapeutic agents was carried out in 47 rats for 5 days. Of them, 23 received chemotherapeutic (Mitomycin C/5-fluorouracil) and 24 received chemoimmunotherapeutic (Mitomycin C/5-fluorouracil/Lentinan) agents. The overall success rate of complete 5-day infusion of the agents was 70.2%. The concentration of 5-fluorouracil in the peripheral blood ranged from 5ng/ml to 17ng/ml (mean 10.4ng/ml) and in the portal blood from 192ng/ml to 610ng/ml (mean 312.0ng/ml) following the perfect continuous infusion of the agents. It can thus be suggested that our established experimental model can be used for the study of continuous intraportal infusion in unrestrained rats.
辅助性门静脉持续化疗在结直肠癌根治术后预防肝转移方面的效果仍不明确。为提高该治疗方法的疗效，我们尝试在雄性Wistar大鼠中建立门静脉持续输注的实验模型。使用可固定在大鼠背部的药物输注球囊导管，对47只大鼠进行了为期5天的门静脉持续化疗/免疫治疗药物给药。其中，23只接受化疗药物（丝裂霉素C/5-氟尿嘧啶），24只接受化疗免疫治疗药物（丝裂霉素C/5-氟尿嘧啶/香菇多糖）。药物完全输注5天的总体成功率为70.2%。在药物完美持续输注后，外周血中5-氟尿嘧啶的浓度范围为5ng/ml至17ng/ml（平均10.4ng/ml），门静脉血中为192ng/ml至610ng/ml（平均312.0ng/ml）。因此，可以认为我们建立的实验模型可用于在不受限制的大鼠中进行门静脉持续输注的研究。
