利用2-羟基-3-萘甲酸-2'-苯胺磷酸酯和固红TR原位杂交检测特定细菌细胞
Detection of specific bacterial cells with 2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate and fast red TR in situ hybridization.
作者信息
Yamaguchi N, Inaoka S, Tani K, Kenzaka T, Nasu M
机构信息
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
出版信息
Appl Environ Microbiol. 1996 Jan;62(1):275-8. doi: 10.1128/aem.62.1.275-278.1996.
Abstract
An in situ hybridization technique with HNPP (2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate) and Fast Red TR was used to detect specific bacterial cells at the single-cell level. By this technique, the fluorescent signals of target bacterial cells were up to eight times more intense than those of standard fluorescence in situ hybridization with mono-fluorescein isothiocyanate-labeled oligonucleotide probes. This novel HNPP-Fast Red TR whole-cell hybridization technique is available for the identification of small or low-rRNA-content bacterial cells in the natural environment.
摘要
采用一种用HNPP（2-羟基-3-萘甲酸-2'-苯胺磷酸酯）和固红TR的原位杂交技术在单细胞水平检测特定细菌细胞。通过该技术，目标细菌细胞的荧光信号强度比用单异硫氰酸荧光素标记的寡核苷酸探针进行的标准荧光原位杂交的信号强度高八倍。这种新型的HNPP-固红TR全细胞杂交技术可用于鉴定自然环境中体积小或rRNA含量低的细菌细胞。
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