苏云金芽孢杆菌δ-内毒素CryIAa第三结构域的定点突变影响其增加家蚕中肠刷状缘膜囊泡通透性的能力。
Site-directed mutations in the third domain of Bacillus thuringiensis delta-endotoxin CryIAa affect its ability to increase the permeability of Bombyx mori midgut brush border membrane vesicles.
作者信息
Wolfersberger M G, Chen X J, Dean D H
机构信息
Department of Biology, Temple University, Philadelphia, Pennsylvania 19122, USA.
出版信息
Appl Environ Microbiol. 1996 Jan;62(1):279-82. doi: 10.1128/aem.62.1.279-282.1996.
Abstract
A series of mutant Bacillus thuringiensis CryIAa delta-endotoxin proteins was prepared by replacing the first, second, and last arginine residues of the conserved third-domain sequence, R-521 YRVRIR-527, with other amino acids. The stable mutant proteins were bioassayed against Bombyx mori larvae and found to all be approximately half as active as wild-type CryIAa. The toxins were also tested by means of a light-scattering assay for their ability to increase permeability of larval B. mori midgut brush border membrane vesicles. Three of the mutant toxins were as active as the wild-type toxin in the vesicle permeability assay.
摘要
通过将保守的第三结构域序列R-521 YRVRIR-527的第一个、第二个和最后一个精氨酸残基替换为其他氨基酸，制备了一系列苏云金芽孢杆菌CryIAaδ-内毒素突变蛋白。对稳定的突变蛋白进行了针对家蚕幼虫的生物测定，发现它们的活性均约为野生型CryIAa的一半。还通过光散射测定法测试了这些毒素增加家蚕幼虫中肠刷状缘膜囊泡通透性的能力。在囊泡通透性测定中，三种突变毒素与野生型毒素活性相同。
相似文献
1
Site-directed mutations in the third domain of Bacillus thuringiensis delta-endotoxin CryIAa affect its ability to increase the permeability of Bombyx mori midgut brush border membrane vesicles.苏云金芽孢杆菌δ-内毒素CryIAa第三结构域的定点突变影响其增加家蚕中肠刷状缘膜囊泡通透性的能力。
Appl Environ Microbiol. 1996 Jan;62(1):279-82. doi: 10.1128/aem.62.1.279-282.1996.
2
Mutations at domain II, loop 3, of Bacillus thuringiensis CryIAa and CryIAb delta-endotoxins suggest loop 3 is involved in initial binding to lepidopteran midguts.苏云金芽孢杆菌CryIAa和CryIAbδ-内毒素结构域II的环3处的突变表明，环3参与了与鳞翅目昆虫中肠的初始结合。
J Biol Chem. 1996 Oct 11;271(41):25220-6. doi: 10.1074/jbc.271.41.25220.
3
Identification of amino acid residues of Bacillus thuringiensis delta-endotoxin CryIAa associated with membrane binding and toxicity to Bombyx mori.苏云金芽孢杆菌δ-内毒素CryIAa中与膜结合及对家蚕毒性相关的氨基酸残基的鉴定
J Bacteriol. 1994 Sep;176(17):5554-9. doi: 10.1128/jb.176.17.5554-5559.1994.
4
Site-directed mutations in a highly conserved region of Bacillus thuringiensis delta-endotoxin affect inhibition of short circuit current across Bombyx mori midguts.苏云金芽孢杆菌δ-内毒素高度保守区域的定点突变影响家蚕中肠短路电流的抑制。
Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9041-5. doi: 10.1073/pnas.90.19.9041.
5
Mutations in domain I interhelical loops affect the rate of pore formation by the Bacillus thuringiensis Cry1Aa toxin in insect midgut brush border membrane vesicles.结构域I螺旋间环中的突变会影响苏云金芽孢杆菌Cry1Aa毒素在昆虫中肠刷状缘膜囊泡中形成孔道的速率。
Appl Environ Microbiol. 2009 Jun;75(12):3842-50. doi: 10.1128/AEM.02924-08. Epub 2009 Apr 17.
6
Location of a Bombyx mori receptor binding region on a Bacillus thuringiensis delta-endotoxin.家蚕受体结合区域在苏云金芽孢杆菌δ-内毒素上的定位
J Biol Chem. 1992 Feb 15;267(5):3115-21.
7
Interaction of the insecticidal crystal protein CryIA from Bacillus thuringiensis with amino acid transport into brush border membranes from Bombyx mori larval midgut.苏云金芽孢杆菌杀虫晶体蛋白CryIA与家蚕幼虫中肠刷状缘膜氨基酸转运的相互作用。
J Invertebr Pathol. 1995 Jan;65(1):35-42. doi: 10.1006/jipa.1995.1005.
8
Purification and partial amino acid sequences of the binding protein from Bombyx mori for CryIAa delta-endotoxin of Bacillus thuringiensis.家蚕中苏云金芽孢杆菌CryIAaδ-内毒素结合蛋白的纯化及部分氨基酸序列
Comp Biochem Physiol B Biochem Mol Biol. 1998 May;120(1):197-204. doi: 10.1016/s0305-0491(98)10009-3.
9
Single-site mutations in the conserved alternating-arginine region affect ionic channels formed by CryIAa, a Bacillus thuringiensis toxin.苏云金芽孢杆菌毒素CryIAa形成的离子通道受保守交替精氨酸区域的单一位点突变影响。
Appl Environ Microbiol. 1997 Oct;63(10):3978-84. doi: 10.1128/aem.63.10.3978-3984.1997.
10
Single amino acid changes in domain II of Bacillus thuringiensis CryIAb delta-endotoxin affect irreversible binding to Manduca sexta midgut membrane vesicles.苏云金芽孢杆菌CryIAbδ-内毒素结构域II中的单氨基酸变化影响其与烟草天蛾中肠膜囊泡的不可逆结合。
J Bacteriol. 1995 May;177(9):2276-82. doi: 10.1128/jb.177.9.2276-2282.1995.
引用本文的文献
1
New insight to structure-function relationship of GalNAc mediated primary interaction between insecticidal Cry1Ac toxin and HaALP receptor of Helicoverpa armigera.关于GalNAc介导的杀虫Cry1Ac毒素与棉铃虫HaALP受体之间初级相互作用的结构-功能关系的新见解。
PLoS One. 2013 Oct 24;8(10):e78249. doi: 10.1371/journal.pone.0078249. eCollection 2013.
2
Differential protection of Cry1Fa toxin against Spodoptera frugiperda larval gut proteases by cadherin orthologs correlates with increased synergism.钙粘蛋白直系同源物对Cry1Fa 毒素对亚洲玉米螟幼虫肠道蛋白酶的差异保护作用与增效作用增强相关。
Appl Environ Microbiol. 2012 Jan;78(2):354-62. doi: 10.1128/AEM.06212-11. Epub 2011 Nov 11.
3
Novel Bacillus thuringiensis δ-endotoxin active against Locusta migratoria manilensis.新型苏云金芽孢杆菌δ-内毒素对东亚飞蝗有效。
Appl Environ Microbiol. 2011 May;77(10):3227-33. doi: 10.1128/AEM.02462-10. Epub 2011 Mar 25.
4
Bacillus thuringiensis: a genomics and proteomics perspective.苏云金芽孢杆菌：基因组学与蛋白质组学视角
Bioeng Bugs. 2010 Jan-Feb;1(1):31-50. doi: 10.4161/bbug.1.1.10519.
5
Influence of mutagenesis of Bacillus thuringiensis Cry1Aa toxin on larvicidal activity.苏云金芽孢杆菌 Cry1Aa 毒素突变对其杀虫活性的影响。
Curr Microbiol. 2011 Mar;62(3):968-73. doi: 10.1007/s00284-010-9791-2. Epub 2010 Nov 17.
6
A Cry1Ac toxin variant generated by directed evolution has enhanced toxicity against Lepidopteran insects.经定向进化产生的 Cry1Ac 毒素变体对鳞翅目昆虫的毒性增强。
Curr Microbiol. 2011 Feb;62(2):358-65. doi: 10.1007/s00284-010-9714-2. Epub 2010 Jul 29.
7
Lepidopteran-active variable-region sequence imparts coleopteran activity in eCry3.1Ab, an engineered Bacillus thuringiensis hybrid insecticidal protein.鳞翅目活性可变区序列赋予了 eCry3.1Ab（一种经过工程改造的苏云金芽孢杆菌混合杀虫蛋白）鞘翅目活性。
Appl Environ Microbiol. 2010 May;76(10):3082-8. doi: 10.1128/AEM.00155-10. Epub 2010 Mar 19.
8
Membrane insertion of the Bacillus thuringiensis Cry1Ab toxin: single mutation in domain II block partitioning of the toxin into the brush border membrane.苏云金芽孢杆菌Cry1Ab毒素的膜插入：结构域II中的单个突变阻止毒素在刷状缘膜中的分配。
Biochemistry. 2008 May 27;47(21):5814-22. doi: 10.1021/bi7014234. Epub 2008 May 6.
9
Comparative study of Bacillus thuringiensis Cry1Aa and Cry1Ac delta-endotoxin activation, inactivation and in situ histopathological effect in Ephestia kuehniella (Lepidoptera: Pyralidae).苏云金芽孢杆菌Cry1Aa和Cry1Acδ-内毒素在粉斑螟（鳞翅目：螟蛾科）中的激活、失活及原位组织病理学效应的比较研究
Mol Biotechnol. 2008 Mar;38(3):233-9. doi: 10.1007/s12033-007-9021-8. Epub 2007 Nov 27.
10
A mechanical force contributes to the "osmotic swelling" of brush-border membrane vesicles.机械力促成刷状缘膜囊泡的“渗透性肿胀”。
Biophys J. 2006 Nov 1;91(9):3301-12. doi: 10.1529/biophysj.106.088641. Epub 2006 Aug 11.
本文引用的文献
1
Site-directed mutations in a highly conserved region of Bacillus thuringiensis delta-endotoxin affect inhibition of short circuit current across Bombyx mori midguts.苏云金芽孢杆菌δ-内毒素高度保守区域的定点突变影响家蚕中肠短路电流的抑制。
Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9041-5. doi: 10.1073/pnas.90.19.9041.
2
An analysis of Bacillus thuringiensis delta-endotoxin action on insect-midgut-membrane permeability using a light-scattering assay.
Eur J Biochem. 1993 Jun 15;214(3):771-8. doi: 10.1111/j.1432-1033.1993.tb17979.x.
3
Functional analysis of block 5, one of the highly conserved amino acid sequences in the 130-kDa CryIVA protein produced by Bacillus thuringiensis subsp. israelensis.苏云金芽孢杆菌以色列亚种产生的130 kDa CryIVA蛋白中高度保守的氨基酸序列之一，即第5区段的功能分析。
FEBS Lett. 1994 Jul 18;348(3):249-54. doi: 10.1016/0014-5793(94)00604-0.
4
Single amino acid changes in domain II of Bacillus thuringiensis CryIAb delta-endotoxin affect irreversible binding to Manduca sexta midgut membrane vesicles.苏云金芽孢杆菌CryIAbδ-内毒素结构域II中的单氨基酸变化影响其与烟草天蛾中肠膜囊泡的不可逆结合。
J Bacteriol. 1995 May;177(9):2276-82. doi: 10.1128/jb.177.9.2276-2282.1995.
5
Rapid and efficient site-specific mutagenesis without phenotypic selection.无需表型筛选的快速高效位点特异性诱变。
Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92. doi: 10.1073/pnas.82.2.488.
6
Insecticidal crystal proteins of Bacillus thuringiensis.苏云金芽孢杆菌的杀虫晶体蛋白
Microbiol Rev. 1989 Jun;53(2):242-55. doi: 10.1128/mr.53.2.242-255.1989.
7
Specificity of Bacillus thuringiensis delta-endotoxins. Importance of specific receptors on the brush border membrane of the mid-gut of target insects.苏云金芽孢杆菌δ-内毒素的特异性。靶标昆虫中肠刷状缘膜上特异性受体的重要性。
Eur J Biochem. 1989 Dec 8;186(1-2):239-47. doi: 10.1111/j.1432-1033.1989.tb15201.x.
8
Location of the Bombyx mori specificity domain on a Bacillus thuringiensis delta-endotoxin protein.家蚕特异性结构域在苏云金芽孢杆菌δ-内毒素蛋白上的定位
Proc Natl Acad Sci U S A. 1989 Jun;86(11):4037-41. doi: 10.1073/pnas.86.11.4037.
9
Hyperexpression of a Bacillus thuringiensis delta-endotoxin-encoding gene in Escherichia coli: properties of the product.
Gene. 1990 Sep 1;93(1):49-54. doi: 10.1016/0378-1119(90)90134-d.
10
Crystal structure of insecticidal delta-endotoxin from Bacillus thuringiensis at 2.5 A resolution.苏云金芽孢杆菌杀虫δ-内毒素的晶体结构，分辨率为2.5埃
Nature. 1991 Oct 31;353(6347):815-21. doi: 10.1038/353815a0.
Zotero 插件