Del Mazo J, McGuigan J E
J Lab Clin Med. 1976 Aug;88(2):292-300.
Specific binding of radiolabeled gastrin by gastric mucosal cells prepared from guinea pigs was detected and examined. Specific cell binding of gastrin was found to be both pH and temperature dependent. Maximum binding of radiolabeled gastrin by gastric mucosal cells was demonstrated at pH 7.4 and at 4 degrees C. Substantial degradation of 125I-gastrin occurred during incubation with gastric mucosal cells. Gastrin degradation was detected both by loss of immunological reactivity with antibodies to gastrin and by abolition of subsequent specific binding after prior incubation with gastric mucosal cells. Degradation of gastrin was most marked with incubations conducted at pH 7.4 and at low pH (pH 2.0), suggesting the activities of at least two gastrin-degrading enzyme systems in the gastric mucosal cell preparation.
检测并研究了从豚鼠制备的胃黏膜细胞对放射性标记胃泌素的特异性结合。发现胃泌素的特异性细胞结合既依赖于pH值，也依赖于温度。胃黏膜细胞对放射性标记胃泌素的最大结合在pH 7.4和4℃时表现出来。在与胃黏膜细胞孵育期间，125I-胃泌素发生了大量降解。通过与胃泌素抗体的免疫反应性丧失以及在与胃黏膜细胞预先孵育后随后特异性结合的消除来检测胃泌素降解。在pH 7.4和低pH（pH 2.0）下进行孵育时，胃泌素降解最为明显，这表明在胃黏膜细胞制剂中至少有两种胃泌素降解酶系统的活性。
