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1
Purification by cobalamin-Sepharose affinity chromatography and intrinsic factor-binding activity of an extramembrane proteolytic product from pig ileal mucosa.通过钴胺素-琼脂糖亲和色谱法对猪回肠黏膜膜外蛋白水解产物进行纯化及其内因子结合活性
Biochem J. 1996 Jan 15;313 ( Pt 2)(Pt 2):675-81. doi: 10.1042/bj3130675.
2
Lectin binding to the porcine and human ileal receptor of intrinsic factor-cobalamin.凝集素与内因子 - 钴胺素的猪和人回肠受体的结合。
Glycoconj J. 1989;6(4):525-38. doi: 10.1007/BF01053775.
3
Purification of intrinsic factor receptor from pig ileum using as affinity medium human intrinsic factor covalently bound to Sepharose.使用共价结合到琼脂糖凝胶上的人内因子作为亲和介质从猪回肠中纯化内因子受体。
Biochim Biophys Acta. 1989 Sep 15;992(3):281-8. doi: 10.1016/0304-4165(89)90086-x.
4
Intrinsic factor-cobalamin accumulates in the ilea of mice treated with chloroquine.内因子-钴胺素在接受氯喹治疗的小鼠回肠中蓄积。
Gastroenterology. 1985 Dec;89(6):1353-9. doi: 10.1016/0016-5085(85)90655-9.
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Release of vitamin B-12 in vitro from intrinsic factor-vitamin B-12 complex. Purification and characterization of a releasing factor from rat ileal brush border.维生素B-12从内因子-维生素B-12复合物中的体外释放。大鼠回肠刷状缘释放因子的纯化与特性鉴定。
Biochim Biophys Acta. 1982 Dec 17;719(3):619-32. doi: 10.1016/0304-4165(82)90253-7.
6
Binding of intrinsic factor to ileal brush border membrane in the rat.大鼠体内内因子与回肠刷状缘膜的结合。
Biochem Biophys Res Commun. 1983 Aug 30;115(1):238-44. doi: 10.1016/0006-291x(83)90995-6.
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Isolation and characterization of proteolytically derived ileal receptor for intrinsic factor-cobalamin.内因子-钴胺素蛋白水解衍生的回肠受体的分离与鉴定
J Biol Chem. 1982 Jan 10;257(1):183-9.
8
Isolation and characterization of the ileal receptor for intrinsic factor-cobalamin.内因子-钴胺素回肠受体的分离与特性研究
J Biol Chem. 1981 Apr 25;256(8):3785-90.
9
Solubilization of the pig ileal intrinsic factor receptor with papain treatment and studies on the solubilized receptor.用木瓜蛋白酶处理溶解猪回肠内因子受体并对溶解后的受体进行研究。
Biochim Biophys Acta. 1980 Nov 20;626(1):244-53. doi: 10.1016/0005-2795(80)90215-9.
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Immunocytochemical localization of intrinsic factor--cobalamin bound to the guinea pig ileum in vivo.体内与豚鼠回肠结合的内因子 - 钴胺素的免疫细胞化学定位。
Gastroenterology. 1982 Feb;82(2):284-90.

本文引用的文献

1
Decreased activity of intestinal and urinary intrinsic factor receptor in Gräsbeck-Imerslund disease [corrected].格里斯贝克-伊默斯隆病中肠道和尿内因子受体活性降低[已修正]
Gastroenterology. 1995 Jun;108(6):1622-8. doi: 10.1016/0016-5085(95)90122-1.
2
Intrinsic factor covalently bound to Sepharose as affinity medium for the purification of a soluble intrinsic factor receptor from human urine.共价结合到琼脂糖凝胶上的内因子作为亲和介质，用于从人尿中纯化可溶性内因子受体。
J Chromatogr B Biomed Appl. 1995 Feb 3;664(1):253-9. doi: 10.1016/0378-4347(94)00426-6.
3
A new solid-state reagent to iodinate proteins. I. Conditions for the efficient labeling of antiserum.一种用于蛋白质碘化的新型固态试剂。I. 高效标记抗血清的条件。
Anal Biochem. 1982 Sep 15;125(2):427-32. doi: 10.1016/0003-2697(82)90025-2.
4
Immunocytochemical localization of the intrinsic factor-cobalamin receptor in dog-ileum: distribution of intracellular receptor during cell maturation.犬回肠中内因子 - 钴胺素受体的免疫细胞化学定位：细胞成熟过程中细胞内受体的分布
J Cell Biol. 1984 Mar;98(3):1111-8. doi: 10.1083/jcb.98.3.1111.
5
Isolation and characterization of proteolytically derived ileal receptor for intrinsic factor-cobalamin.内因子-钴胺素蛋白水解衍生的回肠受体的分离与鉴定
J Biol Chem. 1982 Jan 10;257(1):183-9.
6
The hydrophobic nature of the pig intrinsic factor receptor in the intestine.猪肠道内源性因子受体的疏水性
Biochim Biophys Acta. 1981 Aug 20;646(2):268-73. doi: 10.1016/0005-2736(81)90333-3.
7
Isolation and characterization of the ileal receptor for intrinsic factor-cobalamin.内因子-钴胺素回肠受体的分离与特性研究
J Biol Chem. 1981 Apr 25;256(8):3785-90.
8
Solubilization of the pig ileal intrinsic factor receptor with papain treatment and studies on the solubilized receptor.用木瓜蛋白酶处理溶解猪回肠内因子受体并对溶解后的受体进行研究。
Biochim Biophys Acta. 1980 Nov 20;626(1):244-53. doi: 10.1016/0005-2795(80)90215-9.
9
Carbohydrate binding studies on the lectin from Datura stramonium seeds.曼陀罗种子凝集素的碳水化合物结合研究。
Arch Biochem Biophys. 1984 Jun;231(2):524-33. doi: 10.1016/0003-9861(84)90417-x.
10
Isolation and characterization of two species of receptor which bind intrinsic factor.两种与内因子结合的受体的分离与特性鉴定。
Arch Biochem Biophys. 1984 Dec;235(2):482-92. doi: 10.1016/0003-9861(84)90221-2.

通过钴胺素-琼脂糖亲和色谱法对猪回肠黏膜膜外蛋白水解产物进行纯化及其内因子结合活性

Purification by cobalamin-Sepharose affinity chromatography and intrinsic factor-binding activity of an extramembrane proteolytic product from pig ileal mucosa.

作者信息

Yerima A, Safi A, Gastin I, Michalski J C, Saunier M, Gueant J L

机构信息

Laboratoire de Biologie Cellulaire et Moléculaire en Nutrition et INSERM Unité 308 Faculté de Médecine, Université de Nancy, France.

出版信息

Biochem J. 1996 Jan 15;313 ( Pt 2)(Pt 2):675-81. doi: 10.1042/bj3130675.

DOI:10.1042/bj3130675
PMID:8573109
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1216960/
Abstract

We have purified a cobalamin-binding protein obtained by papain digestion of pig intestine by cobalamin-AH-Sepharose affinity chromatography, with a purification factor of 17,300, a yield of 63% and a cobalamin-binding activity of 11,260 pmol/mg of protein. The protein contained 3.8% carbohydrate and was O- and N-glycosylated. Its molecular mass was 69 kDa on SDS/PAGE and its isoelectric point was 5.1. It had a binding activity for both [57Co]cobalamin and [57Co]cobalamin-intrinsic factor in native PAGE autoradiography and it inhibited the binding of intrinsic factor to the intact intestinal receptor with an IC50 of 49.31 nmol/l in a radioisotope assay. In conclusion, the purified protein shared a binding activity for both cobalamin and intrinsic factor-cobalamin complexes and could correspond to the extracellular domain of the ileal intrinsic factor receptor.

摘要

我们通过钴胺素-AH-琼脂糖亲和色谱法纯化了一种通过木瓜蛋白酶消化猪小肠获得的钴胺素结合蛋白，纯化因子为17300，产率为63%，钴胺素结合活性为11260 pmol/mg蛋白质。该蛋白质含有3.8%的碳水化合物，且存在O-糖基化和N-糖基化。在SDS/PAGE上其分子量为69 kDa，等电点为5.1。在天然PAGE放射自显影中，它对[57Co]钴胺素和[57Co]钴胺素-内因子均具有结合活性，并且在放射性同位素测定中，它抑制内因子与完整肠道受体的结合，IC50为49.31 nmol/l。总之，纯化的蛋白质对钴胺素和内因子-钴胺素复合物均具有结合活性，可能对应于回肠内因子受体的细胞外结构域。