Ali S, Ravindranath N, Jia M C, Musto N, Tsujimura T, Kitamura Y, Dym M
Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA.
Biochem Biophys Res Commun. 1996 Jan 5;218(1):104-12. doi: 10.1006/bbrc.1996.0019.
The c-kit protooncogene is a transmembrane tyrosine kinase receptor expressed during gametogenesis. Using the polymerase chain reaction (PCR), we have identified the c-kit receptor mRNA transcripts in the rat testis and studied their expression during postnatal development of the testis. Five different transcripts were identified using sets of primers encoding within the extracellular domain. Two transcripts were obtained from primer sets encoding regions within the cytoplasmic domain and the primer set encoding the entire length of the c-kit receptor. We have compared the levels of expression of these transcripts on different days during postnatal development. The level of expression of a particular transcript varied depending upon the developmental stage of the testis. In summary, our results suggest that multiple forms of mRNAs exist for the c-kit receptor in the rat testis, and they are regulated differentially during postnatal development.
c-kit原癌基因是一种在配子发生过程中表达的跨膜酪氨酸激酶受体。利用聚合酶链反应（PCR），我们在大鼠睾丸中鉴定出了c-kit受体mRNA转录本，并研究了它们在睾丸出生后发育过程中的表达情况。使用编码细胞外结构域内的引物组鉴定出了五种不同的转录本。从编码细胞质结构域内区域的引物组以及编码c-kit受体全长的引物组中获得了两种转录本。我们比较了这些转录本在出生后发育不同天数的表达水平。特定转录本的表达水平因睾丸的发育阶段而异。总之，我们的结果表明，大鼠睾丸中存在多种形式的c-kit受体mRNA，并且它们在出生后发育过程中受到不同的调节。
