Sugiura T, Kondo S, Sukagawa A, Tonegawa T, Nakane S, Yamashita A, Waku K
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Biochem Biophys Res Commun. 1996 Jan 5;218(1):113-7. doi: 10.1006/bbrc.1996.0020.
Rat testis was shown to contain significant amounts of both N-acylethanolamine, including N-arachidonoylethanolamine (anandamide), and N-acylphosphatidylethanolamine (N-acylPE), including N-arachidonoylPE. The fatty acid profiles of the N-acyl moieties of the two classes resembled each other. We confirmed that testis microsomes contain a phosphodiesterase activity catalyzing the release of anandamide from N-arachidonoylPE. They also contain an enzyme activity catalyzing the transfer of arachidonic acid from the 1-position of diacylphospholipids to PE to form N-arachidonoylPE. These results suggest that the N-acylPE pathway is important in the synthesis of anandamide in this tissue.
研究表明，大鼠睾丸中含有大量的N-酰基乙醇胺，包括N-花生四烯酰乙醇胺（花生四烯酸乙醇胺），以及N-酰基磷脂酰乙醇胺（N-酰基PE），包括N-花生四烯酰PE。这两类物质的N-酰基部分的脂肪酸谱彼此相似。我们证实，睾丸微粒体含有一种磷酸二酯酶活性，可催化从N-花生四烯酰PE中释放花生四烯酸乙醇胺。它们还含有一种酶活性，可催化花生四烯酸从二酰基磷脂的1位转移至PE以形成N-花生四烯酰PE。这些结果表明，N-酰基PE途径在该组织中花生四烯酸乙醇胺的合成中起重要作用。
