Woods T C, Graber J M, Hershow R C, Khabbaz R F, Kaplan J E, Heneine W
Retrovirus Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
AIDS Res Hum Retroviruses. 1995 Oct;11(10):1235-9. doi: 10.1089/aid.1995.11.1235.
Human T-lymphotrophic virus type II (HTLV-II) has not yet been associated with any disease. Little is known about the proviral loads of HTLV-II in vivo and its relationship, if any, to lack of pathogenicity. We determined the HTLV-II proviral copy number in peripheral blood lymphocyte (PBL) samples from 49 HTLV-II-infected individuals, of whom 25 were coinfected with human immunodeficiency virus type 1 (HIV-1). The HTLV-II copy numbers were determined by polymerase chain reaction (PCR) amplification of end-point dilutions of PBL lysates, followed by hybridization to a 32P-labeled HTLV-II-specific probe. The proviral copy number for the 49 samples ranged from < 0.02 to 200 per 1000 PBLs; 6% had < 0.02, 16% had 0.02, 20% had 0.2, 18% had 2, 31% had 20, and 8% had 200 copies per 1000 PBLs. The distributions of HTLV-II copy numbers in the coinfected and singly infected subgroups were not significantly different (Wilcoxon rank sum, p = 0.24). In the coinfected subgroup, there was no significant correlation between the HTLV-II proviral load and the counts of CD4-positive lymphocytes or CD8-positive lymphocytes (Spearman Coefficient = 0.26, p = 0.20; = 0.091, p = 0.67, respectively). Our data demonstrate the presence of a wide range of viral loads in HTLV-II-infected individuals. The high viral loads (> or = 20 copies/1000 lymphocytes) detected in 39% of our samples suggest that the low pathogenicity of HTLV-II is not related to the presence of low viral loads in the infected subjects. Our data from the HIV-1 coinfected individuals show no apparent effect of HIV-1 on HTLV-II proviral loads.
人类嗜T淋巴细胞病毒II型（HTLV-II）尚未与任何疾病相关联。关于HTLV-II在体内的前病毒载量及其与缺乏致病性（若存在关联）的关系，人们了解甚少。我们测定了49例HTLV-II感染者外周血淋巴细胞（PBL）样本中的HTLV-II前病毒拷贝数，其中25例同时感染了1型人类免疫缺陷病毒（HIV-1）。通过对PBL裂解物终点稀释液进行聚合酶链反应（PCR）扩增，随后与32P标记的HTLV-II特异性探针杂交来确定HTLV-II拷贝数。49个样本的前病毒拷贝数范围为每1000个PBL中<0.02至200个；6%的样本<0.02，16%的样本为0.02，20%的样本为0.2，18%的样本为2，31%的样本为20，8%的样本为每1000个PBL中有200个拷贝。同时感染组和单独感染组中HTLV-II拷贝数的分布无显著差异（Wilcoxon秩和检验，p = 0.24）。在同时感染组中，HTLV-II前病毒载量与CD4阳性淋巴细胞计数或CD8阳性淋巴细胞计数之间无显著相关性（Spearman系数分别为0.26，p = 0.20；0.091，p = 0.67）。我们的数据表明，HTLV-II感染者中存在广泛的病毒载量范围。我们39%的样本中检测到的高病毒载量（≥20拷贝/1000淋巴细胞）表明，HTLV-II的低致病性与感染个体中低病毒载量的存在无关。我们来自HIV-1合并感染个体的数据显示，HIV-1对HTLV-II前病毒载量无明显影响。
