Suh H, Lee C
Institute of Biomedical Engineering, College of Medicine, Seoul National University, Korea.
ASAIO J. 1995 Jul-Sep;41(3):M652-6.
To develop an artificial bone substitute that would be gradually degraded and replaced by the regenerating natural bone, a carbonate apatite type I atelocollagen (82/12 in v/v%) composite was designed. The carbonate apatite synthesized at 58 degrees C demonstrated a crystallinity similar to that of natural bone. Type I atelocollagen was purified from human umbilical cords. Carbonate apatite-atelocollagen composite rods (diameter 6 mm x height 10 mm) were irradiated by ultraviolet ray (wave length 254 nm) for 4 hr to increase the collagen fibrillar cross links. Rabbit tibiae were dissected to prepare an artificial total bone defect (length 10 mm). The composites and porous hydroxyapatite rods, sintered at 1200 degrees C, were implanted into the defects and the tibiae were fixed by external osseofixators. The implanted composites were gradually degraded in the lesions, and the regenerated bone totally replaced the defects within 6 weeks, while the hydroxyapatite rod implanted lesions were not replaced by bone. No specific histologic abnormalities appeared in either the hydroxyapatite or composite rod implanted lesions.
为了开发一种能逐渐降解并被再生天然骨替代的人工骨替代物，设计了一种碳酸磷灰石I型去端肽胶原（体积比82/12）复合材料。在58摄氏度合成的碳酸磷灰石显示出与天然骨相似的结晶度。I型去端肽胶原从人脐带中纯化得到。将碳酸磷灰石-去端肽胶原复合棒（直径6毫米×高度10毫米）用紫外线（波长254纳米）照射4小时，以增加胶原纤维的交联。解剖兔胫骨以制备人工全骨缺损（长度10毫米）。将复合材料和在1200摄氏度烧结的多孔羟基磷灰石棒植入缺损处，并用外固定器固定胫骨。植入的复合材料在损伤处逐渐降解，再生骨在6周内完全替代了缺损，而植入羟基磷灰石棒的损伤处未被骨替代。在植入羟基磷灰石或复合棒的损伤处均未出现特定的组织学异常。
