利用肺孢子虫短期培养物进行药物测试。
The use of short-term cultures of Pneumocystis for drug testing.
作者信息
Sahm M, Wingen F, Seitz H M
机构信息
Institut für Medizinische Parasitologie, Universität Bonn Rhône-Poulenc Rorer, Köln.
出版信息
Appl Parasitol. 1996 Jan;37(1):45-53.
A method for short-term drug testing for Pneumocystis is described. Up to now there was no successful cultivation method without feederlayers. Parasites for inocula were obtained from nude rats, kept under conventional conditions. Pneumocystis separation was done by two centrifugation steps from lung homogenates. Cultivation in a modified Tegoshi medium resulted in preservation of parasites for five days, however there was no growth of P. carinii. Quantification was done microscopically by counting trophozoites in triplicate cultures. The percentage of cysts with intracystic bodies did not exceed 2% of parasites. Tests with pentamidine isethionate as a reference drug demonstrated the suitability of this system for drug evaluation.
描述了一种用于肺孢子菌短期药物测试的方法。到目前为止，还没有一种没有饲养层的成功培养方法。接种用的寄生虫取自饲养在常规条件下的裸鼠。通过从肺匀浆中进行两步离心来分离肺孢子菌。在改良的手越培养基中培养可使寄生虫保存五天，但卡氏肺孢子菌没有生长。通过对一式三份培养物中的滋养体进行计数，在显微镜下进行定量分析。含有囊内小体的囊肿百分比不超过寄生虫的2%。以喷他脒异硫氰酸盐作为参考药物进行的测试表明该系统适用于药物评估。
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