Akamizu T, Kohn L D, Mori T
Department of Laboratory Medicine, Kyoto University School of Medicine, Japan.
Endocr J. 1995 Oct;42(5):617-27. doi: 10.1507/endocrj.42.617.
Cloning of TSHR gene and some subsequent studies using the gene were described. Enormous numbers of studies have been performed since the cloning of TSHR gene. Recent molecular studies on TSH receptor and TSHRAb gave various impacts on thyroidology and are resolving past problems. We mainly focused on regulation, processing and glycosylation, TSH- and TSHRAb binding sites, T cell epitopes, and signal transduction of TSHR. Furthermore, we isolated and characterized TSHRAb genes using lymphocytes producing monoclonal TSHRAb obtained from patients with Graves' disease and primary hypothyroidism. Thus, both antigen and antibody genes are cloned. Combined use of these genes will help to investigate the interactions between TSHR and TSHRAb, and may be expected to contribute to the understanding of molecular mechanisms underlying the pathogenesis of autoimmune thyroid disease as well as the physiology of the thyroid gland.
文中描述了促甲状腺激素受体（TSHR）基因的克隆以及随后使用该基因进行的一些研究。自TSHR基因克隆以来，已经开展了大量研究。近期关于促甲状腺激素受体和促甲状腺激素受体抗体（TSHRAb）的分子研究对甲状腺学产生了多方面影响，并正在解决过去的一些问题。我们主要关注TSHR的调控、加工与糖基化、促甲状腺激素（TSH）及TSHRAb结合位点、T细胞表位以及信号转导。此外，我们利用从格雷夫斯病和原发性甲状腺功能减退症患者中获取的产生单克隆TSHRAb的淋巴细胞，分离并鉴定了TSHRAb基因。因此，抗原和抗体基因均已克隆。联合使用这些基因将有助于研究TSHR与TSHRAb之间的相互作用，有望为理解自身免疫性甲状腺疾病发病机制的分子机制以及甲状腺的生理学做出贡献。
