Krugers H J, Medema R M, Postema F, Korf J
Department of Biological Psychiatry, University of Groningen, The Netherlands.
Brain Res. 1995 Oct 23;696(1-2):89-96. doi: 10.1016/0006-8993(95)00783-m.
The aim of this study was (i) to compare the immunocytochemical distribution of the calcium-binding protein calbindin-D28k (CB) in the hippocampus of rats with the pattern of neurodegeneration following adrenalectomy (ADX) using silver impregnation, and (ii) to investigate the CB-immunoreactivity in the hippocampus following 3 weeks corticosterone treatment. 24 h following ADX no degenerative changes, nor alterations in CB-immunoreactivity were found in the hippocampus. Both 3 and 21 days following ADX neurodegeneration in the dentate gyrus could be observed which was accompanied with a loss of CB-immunoreactive (CB-ir) cells in that parts of the dentate gyrus suffering neuronal degeneration. Additionally we observed a marked loss of CB-ir in the CA1 area both 3 and 21 days following ADX. Three weeks daily corticosterone treatment (10 mg/day) induced a marked increase of CB-ir exclusively in the CA1 pyramidal cell layer. We conclude that (i) there is a close relationship between the loss of CB-immunoreactive cells in the DG and the neuronal degeneration in the dentate gyrus following ADX, and (ii) corticosterone appears to be involved in the regulation of calbindin-D28k in the CA1 pyramidal cell layer.
（i）使用银浸染法比较大鼠海马中钙结合蛋白钙结合蛋白-D28k（CB）的免疫细胞化学分布与肾上腺切除术后（ADX）的神经退行性变模式；（ii）研究皮质酮治疗3周后海马中的CB免疫反应性。ADX后24小时，海马中未发现退行性变化，CB免疫反应性也未改变。ADX后3天和21天，均可观察到齿状回的神经退行性变，同时在齿状回发生神经元变性的部分区域，CB免疫反应性（CB-ir）细胞减少。此外，我们还观察到ADX后3天和21天，CA1区的CB-ir明显减少。每天给予皮质酮治疗3周（10毫克/天），仅在CA1锥体细胞层诱导CB-ir显著增加。我们得出结论：（i）ADX后，DG中CB免疫反应性细胞的减少与齿状回的神经元变性密切相关；（ii）皮质酮似乎参与了CA1锥体细胞层中钙结合蛋白-D28k的调节。
