Skullman S, Wirén M, Chu M, Permert J, Garlick P J, McNurlan M A, Larsson J
Department of Surgery, University Hospital, Linköping, Sweden.
Eur J Gastroenterol Hepatol. 1995 Sep;7(9):881-6.
To elucidate whether glutamine can influence the rate of regeneration and protein metabolism in regenerating liver.
Liver regeneration rate, protein content and synthesis were measured in rats 7 days after a liver resection or sham operation. After the operation, the rats were fed three elementary isonitrogenous diets, one without and two including different levels of glutamine.
Fifty-six rats were randomly assigned to either sham operation or liver resection. After the operation, they received an isonitrogenous, isocaloric elementary diet with a glutamine content of 0, 2 or 4%. The resected part of the liver was weighed and analysed for DNA and protein content. Seven days later, hepatic protein synthesis was measured by the flooding method using L-[3H]-phenylalanine, and the liver was analysed for DNA, RNA and protein content.
The regeneration rate was higher in the group receiving 2% glutamine but not in the group receiving 4% glutamine than in the 0% group. Total protein content was increased in regenerating liver in the 2 and 4% glutamine groups compared with the 0% group. Protein synthesis was higher 7 days after liver resection than in sham-operated rats. In the 2% group there was a tendency towards increased protein synthesis compared with the 0% group.
A diet with normal glutamine content improved liver regeneration rate, total protein content and protein synthesis in regenerating liver, but an excess of glutamine did not enhance this effect.
阐明谷氨酰胺是否会影响再生肝脏的再生速率和蛋白质代谢。
在大鼠接受肝切除或假手术后7天，测量其肝脏再生速率、蛋白质含量及合成情况。手术后，给大鼠喂食三种等氮基础日粮，一种不含谷氨酰胺，另外两种含有不同水平的谷氨酰胺。
56只大鼠被随机分为假手术组或肝切除组。手术后，它们接受谷氨酰胺含量分别为0%、2%或4%的等氮、等热量基础日粮。称取肝脏切除部分的重量，并分析其DNA和蛋白质含量。7天后，采用L-[3H]-苯丙氨酸灌流法测量肝脏蛋白质合成，并分析肝脏的DNA、RNA和蛋白质含量。
与0%组相比，接受2%谷氨酰胺的组肝脏再生速率更高，但接受4%谷氨酰胺的组则不然。与0%组相比，2%和4%谷氨酰胺组再生肝脏中的总蛋白含量增加。肝切除术后7天的蛋白质合成高于假手术大鼠。与0%组相比，2%组有蛋白质合成增加的趋势。
含有正常谷氨酰胺含量的日粮可提高再生肝脏的再生速率、总蛋白含量和蛋白质合成，但过量的谷氨酰胺并不能增强这种作用。
