用于从草药中筛选抗病毒药物的鸭乙型肝炎病毒模型

Duck hepatitis B virus model for screening of antiviral agents from medicinal herbs.

作者信息

Mi Z, Chen H, Zhang X, Shao X, Li Z, Wu X

机构信息

Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing.

出版信息

Chin Med J (Engl). 1995 Sep;108(9):660-4.

PMID:8575230

Abstract

The effects of the extracts of 20 Chinese medicinal herbs and an antiviral drug foscarnet on duck hepatitis B virus (DHBV) endogenous DNA polymerase (DNAp) activity were compared. The extracts of P. urinaria showed a dose-dependent inhibition on DHBV DNAp. And those of other herbs showed little inhibition effect. Primary duck hepatocyte (PDH) cultures were used for evaluating effects of the extract of P. urinaria, foscarnet and acyclovir (ACV) on DHBV, and all the drugs or the extracts showed inhibition of DHBV DNA replication. Furthermore, in vivo trials were carried out. Peking ducks infected with LJ-76 strain of DHBV were treated with the extract of P. urinaria or ACV and compared with placebo treated control ducks. The treatment results in the loss or reduction of circulating viral DHBV DNA and DHBsAg.

摘要

比较了20种中草药提取物和抗病毒药物膦甲酸钠对鸭乙型肝炎病毒（DHBV）内源性DNA聚合酶（DNAp）活性的影响。萹蓄提取物对DHBV DNAp表现出剂量依赖性抑制作用。而其他草药提取物的抑制作用很小。原代鸭肝细胞（PDH）培养用于评估萹蓄提取物、膦甲酸钠和阿昔洛韦（ACV）对DHBV的影响，所有药物或提取物均显示出对DHBV DNA复制的抑制作用。此外，还进行了体内试验。用萹蓄提取物或ACV治疗感染DHBV LJ - 76株的北京鸭，并与用安慰剂治疗的对照鸭进行比较。治疗导致循环中的病毒DHBV DNA和DHBsAg减少或消失。