Bhat N R, Zhang P, Bhat A N
Department of Neurology, Medical University of South Carolina, Charleston 29425, USA.
Dev Neurosci. 1995;17(4):256-63. doi: 10.1159/000111294.
Myristoylated alanine-rich C-kinase substrate (MARCKS), a major substrate of activated protein kinase C (PKC), is thought to be involved in PKC-mediated signal transduction events. In the present study, we have examined the expression of MARCKS in primary cultures of rat glial cells. Western blot analysis of different glial cell types (i.e., astrocytes, oligodendrocytes and microglia) revealed a relatively high level of MARCKS protein in oligodendrocytes. MARCKS protein and MARCKS mRNA levels in oligodendrocytes increased with time in culture, indicating a developmental regulation in MARCKS gene expression in differentiating oligodendrocytes. Immunocytochemical examination of developing oligodendrocytes indicated a strong labeling of MARCKS distributed both in the cell body and in the lacy network of processes. These findings, in concert with our previous observations on the role of PKC in oligodendrogenesis, strongly implicate a PKC-signaling system in oligodendrocyte development.
肉豆蔻酰化富含丙氨酸的蛋白激酶C底物（MARCKS）是活化蛋白激酶C（PKC）的主要底物，被认为参与PKC介导的信号转导事件。在本研究中，我们检测了MARCKS在大鼠神经胶质细胞原代培养物中的表达。对不同类型神经胶质细胞（即星形胶质细胞、少突胶质细胞和小胶质细胞）进行的蛋白质免疫印迹分析显示，少突胶质细胞中MARCKS蛋白水平相对较高。少突胶质细胞中MARCKS蛋白和MARCKS mRNA水平随培养时间增加，表明在分化的少突胶质细胞中MARCKS基因表达存在发育调控。对发育中的少突胶质细胞进行免疫细胞化学检查显示，MARCKS在细胞体和呈花边状的突起网络中均有强烈标记。这些发现，与我们之前关于PKC在少突胶质细胞生成中的作用的观察结果一致，强烈表明PKC信号系统参与少突胶质细胞发育。
