Chan T S, Lin C X, Chan W Y, Chung S S, Chung S K
Institute of Molecular Biology, University of Hong Kong.
Eur J Biochem. 1995 Dec 15;234(3):819-26. doi: 10.1111/j.1432-1033.1995.819_a.x.
Endothelin-1 (ET-1) is a peptide implicated in a wide variety of functions involving vascular and non-vascular systems. We have cloned the cDNA encoding the mouse prepro-endothelin-1 (PPET-1) and determined its nucleotide sequence. The putative PPET-1 peptide processing sites are all conserved and the deduced 21-amino-acid mature ET-1 peptide is identical to that of the rat, human, bovine, porcine and rabbit. Using the cloned cDNA as a probe for in situ hybridization, we detected PPET-1 mRNA in different tissues at different stages of mouse embryonic development. Embryos at a stage as early as 9.5 days postcoitum (E9.5) have very strong expression in the branchial epithelium, optic vesicle and the endothelial cells of large blood vessels, including the dorsal aorta and aortic arches. While the expression level in the branchial epithelium was decreasing towards the later stage of embryogenesis, the expression in the endothelial cells increased with age. At E10.5, PPET-1 mRNA was also detected in the otic vesicle as well as in the developing gut epithelium. At later stage of development, the expression of PPET-1 was primarily found in the vascular endothelial cells, cochlea, eye and the gut, with the highest level of PPET-1 mRNA in the endothelial cells of the lung. These data will be useful for analyzing the function of ET-1 in these organs.
内皮素-1（ET-1）是一种参与多种涉及血管和非血管系统功能的肽。我们克隆了编码小鼠前内皮素原-1（PPET-1）的cDNA并确定了其核苷酸序列。推测的PPET-1肽加工位点均保守，推导的21个氨基酸的成熟ET-1肽与大鼠、人、牛、猪和兔的相同。使用克隆的cDNA作为原位杂交探针，我们在小鼠胚胎发育不同阶段的不同组织中检测到PPET-1 mRNA。早在胚泡期9.5天（E9.5）的胚胎在鳃上皮、视泡以及包括背主动脉和主动脉弓在内的大血管内皮细胞中有非常强的表达。虽然鳃上皮中的表达水平在胚胎发育后期下降，但内皮细胞中的表达随年龄增加。在E10.5时，在耳泡以及发育中的肠上皮中也检测到PPET-1 mRNA。在发育后期，PPET-1的表达主要在血管内皮细胞、耳蜗、眼睛和肠道中发现，肺内皮细胞中PPET-1 mRNA水平最高。这些数据将有助于分析ET-1在这些器官中的功能。
