Wang Hongyu, Quan Jiexia, Kotake-Nara Ei-Ichi, Uchide Tsuyoshi, Andoh Tadashi, Saida Kaname
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566 Japan.
Exp Biol Med (Maywood). 2006 Jun;231(6):709-12.
The presence of endothelin (ET)-like immunoreactivity and the cardiovascular effects of mammalian ET-1 in fish have been reported. To identify ET-related peptides in fish, we screened the cDNA library of the salmon (Oncorhynchus keta) stomach by means of rapid amplification of cDNA ends, and we cloned cDNAs encoding an ET-related peptide. The salmon ET-related sequence of 21 amino acids is identical to the trout ET-1 peptide recently purified from kidney specimens of Oncorhynchus mykiss. The deduced amino acid sequence of salmon pre-proET-1 (PPET-1) comprises 244 amino acids, including a putative signal sequence and mature ET-1, as well as big ET-1 and ET-1-like sequences. This precursor, the first reported PPET-1 sequence for Salmoniformes, Teleostei, has low homology with the sequences of human, mouse, frog (Xenopus laevis), and zebrafish (Danio rerio) PPET-1 (26%, 29%, 24%, and 39%, respectively).
已有报道称鱼类体内存在内皮素（ET）样免疫反应性以及哺乳动物ET-1对鱼类心血管系统的影响。为了鉴定鱼类中与ET相关的肽，我们通过cDNA末端快速扩增技术筛选了鲑鱼（Oncorhynchus keta）胃的cDNA文库，并克隆了编码一种与ET相关肽的cDNA。鲑鱼中21个氨基酸的ET相关序列与最近从虹鳟（Oncorhynchus mykiss）肾脏标本中纯化出的鳟鱼ET-1肽相同。推导的鲑鱼前体前内皮素-1（PPET-1）氨基酸序列包含244个氨基酸，包括一个假定的信号序列、成熟的ET-1，以及大内皮素-1和ET-1样序列。这种前体是首次报道的鲑形目硬骨鱼纲的PPET-1序列，与人类、小鼠、青蛙（非洲爪蟾）和斑马鱼（Danio rerio）的PPET-1序列具有较低的同源性（分别为26%、29%、24%和39%）。
