Ono T, Muto S, Matsumoto T, Mochii M, Eguchi G
Faculty of Agriculture, Shinshu University, Ina, Japan.
Exp Anim. 1995 Oct;44(4):275-8. doi: 10.1538/expanim.44.275.
During early stages in avian embryogenesis primordial germ cells (PGCs) show a unique migration pathway toward the gonadal anlage through the circulation. In the present study, liposomes consisting of plasmid DNA (pMiwZ; containing lacZ as a reporter) and Lipofectin were injected into the marginal veins of quail embryos during the stages PGCs were circulating in the blood vessels. The lacZ expression was then histochemically detected in the gonads at later embryonic stages, indicating the expression of the injected DNA in PGCs.
在鸟类胚胎发生的早期阶段，原始生殖细胞（PGCs）通过循环系统向性腺原基呈现出独特的迁移途径。在本研究中，在PGCs在血管中循环的阶段，将由质粒DNA（pMiwZ；含有lacZ作为报告基因）和脂质体转染试剂组成的脂质体注射到鹌鹑胚胎的边缘静脉中。然后在胚胎后期通过组织化学方法在性腺中检测到lacZ表达，这表明注入的DNA在PGCs中表达。
