Song Y, Yao G, Fu C
Department of Ophthalmology, 2nd Clinical College, Norman Bethune University of Medical Sciences, Changchun, China.
Yan Ke Xue Bao. 1995 Mar;11(1):22-5.
To study retinal S-antigen expression in human retinoblastoma and assess if there is a correlation between S-antigen immunoactivity and degree of retinoblastoma cell differentiations.
Ten cases of Chinese retinoblastoma parafin-embedded tissues were applied for this study. A strain of monoclonal antibody,MabA9C6, which defines an epitope in S-antigen retained in fixed-tissue sections, was used to study S-antigen expression in 10 cases of retinoblastomas. S-antigen was localized by the biotinavidin indirect immunoperoxidase technique and purified MabA9C6 ascites fluid was used with 1:100 dilution. The whole procedure could be finished within a few hours.
The S-antigen immunoactivity was observed in different patterns: the "normal" photoreceptor elements incorporated in 3 cases of growing tumors; 3 of 4 Fleurettes and E-W rosettes; and scattered tumor cells in 50% of the cases.
The result suggests that the expression of S-antigen in retinoblastoma may be used to assess the degree of tumor differentiation as another tumor marker in retinoblastoma.
研究视网膜S抗原在人视网膜母细胞瘤中的表达，并评估S抗原免疫活性与视网膜母细胞瘤细胞分化程度之间是否存在相关性。
本研究采用10例中国视网膜母细胞瘤石蜡包埋组织。使用一种单克隆抗体MabA9C6，该抗体可识别固定组织切片中S抗原的一个表位，用于研究10例视网膜母细胞瘤中S抗原的表达。采用生物素-抗生物素蛋白间接免疫过氧化物酶技术定位S抗原，使用纯化的MabA9C6腹水液，稀释比例为1:100。整个过程可在数小时内完成。
观察到S抗原免疫活性呈现不同模式：在3例生长肿瘤中可见“正常”光感受器成分；在4个菊形团和E-W玫瑰花结中的3个中可见；在50%的病例中可见散在的肿瘤细胞。
结果表明，视网膜母细胞瘤中S抗原的表达可作为评估肿瘤分化程度的指标，作为视网膜母细胞瘤的另一种肿瘤标志物。
