Kiassov A P, Van Eyken P, van Pelt J F, Depla E, Fevery J, Desmet V J, Yap S H
Department of Internal Medicine, Laboratory of Hepatology, University of Leuven, Belgium.
Differentiation. 1995 Nov;59(4):253-8. doi: 10.1046/j.1432-0436.1995.5940253.x.
The expression and cellular distribution of desmin, alpha-smooth muscle actin (A-SMA) and cytokeratin no. 8 (CK-8) and no. 18 (CK-18) in normal adult, neonatal and fetal rat liver were examined immunohistochemically on cryostat sections. At days 14 and 15 of gestation, nonhematopoietic cells in embryonic liver were strongly desmin-positive, and some of the cells, mainly located in the periphery, were also stained with anti-A-SMA. Desmin immunoreactivity gradually decreased from day 16 of gestation. A close association of desmin-positive cell processes with hematopoietic cells was observed during fetal and early neonatal development. From day 16 of gestation the pre-hepatocytes became desmin-negative, remained CK-8 and CK-18 positive. Desmin-expressing cells were numerous in the liver from the embryonic period to the neonatal age. However, their absolute number per unit area, as well as their number relative to hepatocytes, decreased with age. We suggest that desmin-positive cells in embryonic liver may act as stromal cells in the hepatic hematopoietic microenvironment and support hepatocyte development.
应用免疫组织化学方法，在低温恒温器切片上检测了结蛋白、α-平滑肌肌动蛋白（A-SMA）、细胞角蛋白8（CK-8）和细胞角蛋白18（CK-18）在正常成年、新生和胎鼠肝脏中的表达及细胞分布。在妊娠第14和15天，胚胎肝脏中的非造血细胞结蛋白呈强阳性，部分细胞（主要位于周边）抗A-SMA染色也呈阳性。从妊娠第16天起，结蛋白免疫反应性逐渐降低。在胎儿期和新生儿早期发育过程中，观察到结蛋白阳性细胞突起与造血细胞密切相关。从妊娠第16天起，前体细胞结蛋白呈阴性，但CK-8和CK-18仍呈阳性。从胚胎期到新生儿期，肝脏中表达结蛋白的细胞数量众多。然而，其单位面积的绝对数量以及相对于肝细胞的数量均随年龄增长而减少。我们认为胚胎肝脏中结蛋白阳性细胞可能作为肝脏造血微环境中的基质细胞，支持肝细胞发育。
