Da Lage J L, Klarenberg A, Cariou M L
UPR Population, Génétique et Evolution, CNRS, Gif sur Yvette, France.
Heredity (Edinb). 1996 Jan;76 ( Pt 1):9-18. doi: 10.1038/hdy.1996.2.
Expression of the amylase multigene family of Drosophila ananassae was investigated in third-instar larvae and adults. A developmental differentiation was found between the Amy1-2 and Amy3-4 gene clusters, the former being preferentially expressed in larvae, the latter in adults. During adult life, we observed a decrease in Amy1-2 expression in males of certain strans. We have raised some arguments for the existence of trans-active regulators, acting as repressors of Amy1-2 in adults. The putative repressors might exhibit a geographical polymorphism, with a fixed active form in Pacific regions and a polymorphic pattern in Africa, thus increasing the diversity observed in adult amylase phenotypes. A clear differentiation between the two gene clusters was also found in tissue-specific activity along the third-instar larval midgut. In the anterior midgut, only Amy1-2 is active, while both gene groups are expressed in the posterior midgut, with an additional subzonation within it.
对拟果蝇淀粉酶多基因家族在三龄幼虫和成虫中的表达进行了研究。发现Amy1-2和Amy3-4基因簇之间存在发育分化，前者在幼虫中优先表达，后者在成虫中优先表达。在成虫期，我们观察到某些品系雄性中Amy1-2的表达下降。我们提出了一些关于反式作用调节因子存在的论据，这些调节因子在成虫中作为Amy1-2的阻遏物起作用。假定的阻遏物可能表现出地理多态性，在太平洋地区有固定的活性形式，在非洲有一个多态模式，从而增加了在成虫淀粉酶表型中观察到的多样性。在三龄幼虫中肠的组织特异性活性方面，也发现了两个基因簇之间的明显分化。在前肠中部，只有Amy1-2是活跃的，而两个基因组在后肠中部都有表达，并且在后肠中部内还有额外的分区。
