Nelson D J, Bugge C, Krasny H C
Adv Exp Med Biol. 1977;76A:121-8. doi: 10.1007/978-1-4613-4223-6_16.
A variety of oxypurines and 6-thiopurines could be transformed by intact erythrocytes to their nucleoside triphosphate forms when incubations were extended for up to 24 hrs. The specific nucleotide monophosphate kinases which accomplish these reactions in erythrocytes were not identified but their ability to utilize 6-thioIMP, 6-thioXMP and 6-methylthioGMP as substrates, albeit very slowly, is clearly implied by these results. S-methylation of 6-thiopurines was demonstrated in erythrocytes incubated with physiological amounts of methionine-(CH3-3H). 6-Methylthioguanosine triphosphate and 6-methylmercaptopurine riboside triphosphate were formed in micromolar amounts, probably from the corresponding thiopurine nucleotides by methyl transfer from S-adenosylmethionine.
当孵育时间延长至24小时时，多种氧嘌呤和6-硫嘌呤可被完整的红细胞转化为其三磷酸核苷形式。虽然尚未鉴定出在红细胞中完成这些反应的特定核苷酸单磷酸激酶，但这些结果清楚地表明它们有能力将6-硫代肌苷一磷酸、6-硫代黄苷一磷酸和6-甲基硫代鸟苷一磷酸作为底物，尽管速度非常缓慢。在用生理量的甲硫氨酸-（CH3-3H）孵育的红细胞中，证明了6-硫嘌呤的S-甲基化。形成了微摩尔量的6-甲基硫代鸟苷三磷酸和6-甲基巯基嘌呤核苷三磷酸，可能是相应的硫嘌呤核苷酸通过S-腺苷甲硫氨酸的甲基转移形成的。
