一种用于检测类病毒的简单印记杂交方法。
A simple imprint-hybridization method for detection of viroids.
作者信息
Romero-Durbán J, Cambra M, Duran-Vila N
机构信息
Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Moncada (Valencia), Spain.
出版信息
J Virol Methods. 1995 Sep;55(1):37-47. doi: 10.1016/0166-0934(95)00043-t.
An imprint-hybridization method has been designed to simplify the processing of samples during routine viroid indexing. The method requires minimal sample manipulation and has been evaluated for detection of viroids in 11 viroid-host combinations including 4 viroids (CEVd, CSVd, HSVd, ASBVd) and 7 hosts (chrysanthemum, citron, cucumber, Gynura, tomato, peach and avocado). The method is fast and sensitive, and provides additional information on the sites of viroid accumulation.
一种印记杂交方法已被设计出来，以简化常规类病毒检测过程中的样本处理。该方法所需的样本操作极少，并且已针对11种类病毒-宿主组合中的类病毒检测进行了评估，其中包括4种类病毒（柑橘裂皮类病毒、黄瓜绿斑驳花叶病毒、马铃薯纺锤块茎类病毒、鳄梨日斑类病毒）和7种宿主（菊花、枸橼、黄瓜、紫背天葵、番茄、桃子和鳄梨）。该方法快速且灵敏，并提供了有关类病毒积累位点的额外信息。
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