Russell L
Am J Anat. 1977 Mar;148(3):313-28. doi: 10.1002/aja.1001480303.
The progressive movement of primary spermatocytes from the basal to the adluminal compartment of the seminiferous tubule was studied after testes were fixed with standard and hypertonic solutions. In stages VI, VII and VIII of the cycle (classification of Leblond and Clermont, '52), preleptotene spermatocytes were observed within the basal compartment of the seminiferous tubule. Resting on the basal lamina, these cells were bound tightly to neighboring Sertoli cells by desmosome-like junctions. In late stage VIII and early stage IX, basal processes of Sertoli cells were observed between the newly formed leptotene cells and the basal lamina, and in stage IX, the Sertoli processes met to form a junction of the zonula adherens type. This junction formed a permeability barrier which restricted the free access of fixative into the spaces around leptotene cells. Evidence for this was found in the absence of the shrinkage artifact produced with hypertonic solutions in earlier stages. In longitudinal sections, the permeability barrier was first observed in an area of the tubule in which sperm release was also taking place. In mid-stage IX and in stage X, sertoli-Sertoli junctional specializations formed de novo below the leptotene spermatocyte, while those from the preceding stages, present above the leptotene spermatocytes, remained intact. Thus, tight junctions were in evidence for a considerable period of the time, both above and below the leptotene spermatocytes. At no time in the process of germ cell movement toward the lumen did these cells exhibit evidence of amoeboid movement or lose desmosome-like contacts with the surrounding Sertoli cells. From this study it is concluded that the Sertoli cells play an active role in the transfer of spermatocytes to the adluminal compartment. A transient intermediate compartment of the seminiferous tubule is described, one which allows for the continual maintenance of the blood-testis barrier during transit of spermatocytes from the basal to the adluminal compartment.
在用标准溶液和高渗溶液固定睾丸后，研究了初级精母细胞在生精小管中从基底区向近腔区的渐进性移动。在周期的VI、VII和VIII阶段（Leblond和Clermont，1952年的分类），在生精小管的基底区内观察到前细线期精母细胞。这些细胞位于基膜上，通过类桥粒连接紧密地与相邻的支持细胞相连。在VIII期末和IX期初，在新形成的细线期细胞和基膜之间观察到支持细胞的基底突起，在IX期，支持细胞的突起相遇形成紧密连接类型的连接。这种连接形成了一个渗透屏障，限制了固定剂自由进入细线期细胞周围的空间。早期阶段用高渗溶液未产生收缩假象，这一现象证明了这一点。在纵切面上，首先在精子也在释放的小管区域观察到渗透屏障。在IX期中期和X期，支持细胞-支持细胞连接特化在细线期精母细胞下方重新形成，而在细线期精母细胞上方的前一阶段的连接特化保持完整。因此，在细线期精母细胞上方和下方相当长的一段时间内都有紧密连接。在生殖细胞向管腔移动的过程中，这些细胞从未表现出变形运动的迹象，也没有失去与周围支持细胞的类桥粒接触。从这项研究得出的结论是，支持细胞在将精母细胞转移到近腔区中起积极作用。描述了生精小管的一个短暂的中间区，在精母细胞从基底区向近腔区转运过程中，该区域允许血睾屏障持续维持。
