肝细胞生长因子可逆转转化生长因子β诱导的CCL-64细胞生长抑制。一种针对肝细胞生长因子的新型生物测定法及其对转化生长因子β生物测定法的意义。

Hepatocyte growth factor reverses the TGF-beta-induced growth inhibition of CCL-64 cells. A novel bioassay for HGF and implications for the TGF-beta bioassay.

作者信息

Börset M, Waage A, Sundan A

机构信息

Institute of Cancer Research and Molecular Biology, University of Trondheim, Norway.

出版信息

J Immunol Methods. 1996 Jan 16;189(1):59-64. doi: 10.1016/0022-1759(95)00228-6.

DOI:10.1016/0022-1759(95)00228-6

PMID:8576580

Abstract

The influence of human hepatocyte growth factor (HGF) on the transforming growth factor beta (TGF-beta) bioassay CCL-64 was examined. HGF induced proliferation of the CCL-64 cells and potently counteracted TGF-beta-induced growth inhibition. HGF was not inactivated by transient acidification to pH 2, a commonly used procedure to activate latent TGF-beta. HGF was a stronger mitogen for the mink lung cells than epidermal growth factor (EGF), a known stimulator of CCL-64 cell growth. Costimulation of the cells by these two cytokines resulted in an additive effect on proliferation. In complex biological fluids containing large amounts of HGF, the TGF-beta concentration can be underestimated when determined by the CCL-64 assay. When a fixed amount of TGF-beta is added, the CCL-64 cells can be used as a reliable bioassay for HGF with a sensitivity of about 1 ng/ml.

摘要

研究了人肝细胞生长因子（HGF）对转化生长因子β（TGF-β）生物测定法CCL-64的影响。HGF诱导CCL-64细胞增殖，并有效对抗TGF-β诱导的生长抑制。通过短暂酸化至pH 2（一种常用的激活潜伏TGF-β的方法），HGF不会失活。HGF对貂肺细胞的促有丝分裂作用比表皮生长因子（EGF，一种已知的CCL-64细胞生长刺激因子）更强。这两种细胞因子对细胞的共同刺激导致增殖产生相加效应。在含有大量HGF的复杂生物流体中，通过CCL-64测定法测定时，TGF-β浓度可能会被低估。当添加固定量的TGF-β时，CCL-64细胞可用作HGF的可靠生物测定法，灵敏度约为1 ng/ml。