Murty B S, Kapoor J N, Kim M W
Am J Hosp Pharm. 1977 Mar;34(3):305-6.
A sensitive, reproducible spectrophotometric procedure using Ellman's reagent for the determination of acetylcysteine in pharmaceutical dosage froms is presented. The method is based on the measurement of the absorbance of the anion of 2-nitro-5-thiolbenzoic acid at 412 nm, liberated as a result of the reaction of acetylcysteine and an alkaline solution of 5-5'-dithiobis-(2-nitrobenzoic acid). The optimum conditions for the reaction, speed of reaction and linearity relationship between concentration and absorbance are reported. It is concluded that the proposed method is simple, rapid and accurate for the determination of small quantities of acetylcysteine.
介绍了一种使用埃尔曼试剂测定药物剂型中乙酰半胱氨酸的灵敏、可重现的分光光度法。该方法基于测量2-硝基-5-硫代苯甲酸阴离子在412nm处的吸光度，此阴离子是乙酰半胱氨酸与5,5'-二硫代双(2-硝基苯甲酸)碱性溶液反应释放出来的。报告了反应的最佳条件、反应速度以及浓度与吸光度之间的线性关系。得出的结论是，所提出的方法对于测定少量乙酰半胱氨酸而言简单、快速且准确。
