Bartsch G, Wagner I, Rohr H P
Andrologia. 1977 Jan-Mar;9(1):79-88. doi: 10.1111/j.1439-0272.1977.tb01266.x.
The alterations induced in principal cells of the epididymal head tissue of the rat by administration of a synthetic progestine (R2323, ethinyl-norgestrienone) were studied by electron microscopy. A total of 3 male, sex mature rats was applicated ethinyl-norgestrienone during a 3 months period in low dosage (60 mug) per day and was studied with 5 control animals. Stereological methods devised by Weibel (1969) and Rohr et al. (1976) were applied to light and electron micrographs. The calculated values are related to 1 cm3 of epididymal tissue, 1 cm3 of epididymal cell and 1 cm3 of epididymal cell cytoplasm. The Golgi apparatus was further subdivided; the values for the Golgi compartments are therefore refered to 1 cm3 of Golgi apparatus was further subdivided; the values for the Golgi compartments are therefore refered to 1 cm3 of Golgi apparatus. In the treated animals there was, related to the unit of epididymal tissue, a significant decrease in the volume density of the interductular tissue, whereas that for the lumina was significantly higher. The volume density of the glandular epithelium remains unchanged. Refered to the unit volume of principal cell cytoplasm the rough endoplasmic reticulum shows a signficant decrease by 6.5%, whereas the smooth endoplasmic retiuclum is increased by 6.3%. The volume density of the lysosomes was calculated to be 4.3% (Control 2.5%). The stereological data of the Golgi apparatus indicate a vacuolar transformation: Increased volume of vacuoles, decreased volume fraction of saccules and vesicles. However, the volume density of the whole Golgi apparatus remains unchanged. An impaired secretory activity of the principal cells could be assumed.
通过电子显微镜研究了给予合成孕激素（R2323，炔诺孕烯酮）对大鼠附睾头部组织主细胞的影响。总共3只性成熟雄性大鼠在3个月期间每天给予低剂量（60微克）炔诺孕烯酮，并与5只对照动物进行研究。应用了Weibel（1969年）和Rohr等人（1976年）设计的体视学方法对光镜和电镜照片进行分析。计算值与1立方厘米的附睾组织、1立方厘米的附睾细胞和1立方厘米的附睾细胞胞质相关。高尔基体进一步细分；因此，高尔基体各部分的值是指1立方厘米的高尔基体。在处理过的动物中，与附睾组织单位相关，管间组织的体积密度显著降低，而管腔的体积密度显著升高。腺上皮的体积密度保持不变。以主细胞胞质单位体积计，粗面内质网显著减少6.5%，而滑面内质网增加6.3%。溶酶体的体积密度计算为4.3%（对照为2.5%）。高尔基体的体视学数据表明存在空泡化转变：空泡体积增加，扁平囊和小泡的体积分数减少。然而，整个高尔基体的体积密度保持不变。可以推测主细胞的分泌活性受损。
