Ishida K, Yamashita H, Katagiri H, Oka Y
Pharmaceutical Section, HOYA Corporation, Saitama, Japan.
Jpn J Ophthalmol. 1995;39(3):225-32.
The regulation mechanism of glucose transporter 1 (GLUT1) gene expression was investigated in cultured bovine corneal endothelial cells. Epidermal growth factor (EGF) stimulated both GLUT1 mRNA expression and cell proliferation. Fetal bovine serum, in contrast, stimulated cell proliferation but did not increase GLUT1 mRNA. IGF-I, hyaluronic acid or fibronectin did not stimulate GLUT1 gene expression. These results suggest that GLUT1 gene expression is controlled, at least in part, by EGF and is related to neither cell proliferation, cell motility nor cell adhesion regulation. The EGF signal was transduced through a pathway including protein kinase C. GLUT1 is reported to work as a water transporter, and so EGF can be considered as one of the possible agent which stimulate water transport activity through the corneal endothelial cells. EGF could be useful in the therapy of corneal edema caused by endothelial dysfunction.
在培养的牛角膜内皮细胞中研究了葡萄糖转运蛋白1（GLUT1）基因表达的调控机制。表皮生长因子（EGF）刺激GLUT1 mRNA表达和细胞增殖。相比之下，胎牛血清刺激细胞增殖，但不增加GLUT1 mRNA。胰岛素样生长因子-I、透明质酸或纤连蛋白不刺激GLUT1基因表达。这些结果表明，GLUT1基因表达至少部分受EGF控制，且与细胞增殖、细胞运动性或细胞黏附调节无关。EGF信号通过包括蛋白激酶C的途径转导。据报道，GLUT1作为一种水转运蛋白发挥作用，因此EGF可被视为通过角膜内皮细胞刺激水转运活性的可能因子之一。EGF可能对治疗由内皮功能障碍引起的角膜水肿有用。
