Krovacek K, Dumontet S, Eriksson E, Baloda S B
Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Biomedical Center, Uppsala, Sweden.
Microbiol Immunol. 1995;39(9):655-61. doi: 10.1111/j.1348-0421.1995.tb03253.x.
A case of food poisoning outbreak involving Aeromonas hydrophila is reported in this study. A group of 27 people consumed a typical Swedish food "landgång" which is a type of smörgåsbord containing shrimps with mayonnaise, liver paté, ham, sausage, and legume salad which was purchased from a food store. Twenty-two of the 27 persons became ill within 20-34 hr of consumption of the food and reported the symptoms ranging from severe acute diarrhea, abdominal pain, headache, fever and vomiting. One person also fainted. The symptoms lasted for a couple of days. Of the remaining 5 healthy persons who consumed the left-over food the next day, 2 became ill with similar symptoms. The bacteriological examination of left-over food samples resulted in the isolation of A. hydrophila from shrimps with mayonnaise, smoked sausage, liver paté and boiled ham. The total number of A. hydrophila in these foods were log 10(6) to log > 10(7) organisms per gram of food sample. A. hydrophila was however, not isolated from legume/mayonnaise salad samples. All the food samples tested showed low numbers of other expected food contaminating organisms such as coliforms at 37 C and 44 C, fecal streptococci, Staphylococcus aureus, fungi and yeast etc., while Bacillus cereus, Clostridium perfringens and Salmonella spp. were not detected in the food samples. Investigations of the virulence profiles of the A. hydrophila isolates showed their capacity to produce beta-hemolysin, cytotoxins, cytotonic toxins, enterotoxins, and adhesion to and invasion of human intestinal (Henle 407) cells in culture.
本研究报告了一起由嗜水气单胞菌引起的食物中毒暴发事件。一组27人食用了一种典型的瑞典食物“landgång”，这是一种包含搭配蛋黄酱的虾、肝酱、火腿、香肠和豆类沙拉的自助餐，该食物从一家食品店购买。27人中的22人在食用该食物后的20至34小时内发病，报告的症状包括严重的急性腹泻、腹痛、头痛、发烧和呕吐。有一人还昏厥了。这些症状持续了几天。在第二天食用剩余食物的其余5名健康人员中，有2人出现了类似症状。对剩余食物样本的细菌学检查结果显示，在搭配蛋黄酱的虾、烟熏香肠、肝酱和煮火腿中分离出了嗜水气单胞菌。这些食物中嗜水气单胞菌的总数为每克食物样本10的6次方至大于10的7次方个菌落形成单位。然而，在豆类/蛋黄酱沙拉样本中未分离出嗜水气单胞菌。所有测试的食物样本显示，其他预期的食物污染微生物数量较少，如37℃和44℃下的大肠菌群、粪链球菌、金黄色葡萄球菌、真菌和酵母菌等，而食物样本中未检测到蜡样芽孢杆菌、产气荚膜梭菌和沙门氏菌属。对嗜水气单胞菌分离株的毒力谱研究表明，它们能够产生β-溶血素、细胞毒素、细胞紧张性毒素、肠毒素，并能在培养中黏附和侵入人肠道（Henle 407）细胞。
