溶组织内阿米巴核糖体RNA转录起始位点的鉴定与分析。

Identification and analysis of the start site of ribosomal RNA transcription of Entamoeba histolytica.

作者信息

Michel B, Lizardi P M, Alagón A, Zurita M

机构信息

Departamento de Genética y Fisiología Molecular, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.

出版信息

Mol Biochem Parasitol. 1995 Jul;73(1-2):19-30. doi: 10.1016/0166-6851(95)00084-e.

DOI:10.1016/0166-6851(95)00084-e

PMID:8577327

Abstract

In this article we report the identification of the start site of ribosomal RNA transcription unit of the enteric parasite E. histolytica. We cloned the upstream region of the ribosomal RNA and we defined the 5' boundary of the transcription unit with nuclear run-on assays. We report that ribosomal transcription starts 2447 bp upstream the SSU ribosomal gene, at an adenosine residue. This data was supported both by S1 mapping and by primer extension analysis; that the mapped site was indeed the transcription start point was demonstrated by RNAse protection of the in vitro capped RNA. Our sequence data around the transcription start point shows two different tandem repeat clusters immediately downstream from the transcription start point.

摘要

在本文中，我们报告了对肠道寄生虫溶组织内阿米巴核糖体RNA转录单元起始位点的鉴定。我们克隆了核糖体RNA的上游区域，并通过细胞核连续转录分析确定了转录单元的5'边界。我们报告核糖体转录在小亚基核糖体基因上游2447 bp处的一个腺苷残基处起始。该数据得到了S1图谱分析和引物延伸分析的支持；通过对体外加帽RNA的RNA酶保护实验证明了所定位的位点确实是转录起始点。我们在转录起始点周围的序列数据显示，在转录起始点下游紧接着有两个不同的串联重复序列簇。