Joppa M A, Meisel R L, Garber M A
Department of Psychological Sciences, Purdue University, West Lafayette, IN 47907-1364, USA.
Neuroscience. 1995 Oct;68(3):783-92. doi: 10.1016/0306-4522(95)00179-m.
The goal of these experiments was to use c-Fos immunocytochemistry to determine areas of the female hamster brain that are active during lordosis and aggression. Ovariectomized hamsters were given (i) estradiol and progesterone, plus a lordosis test, (ii) estradiol and progesterone, but no lordosis test, (iii) oil, plus an aggressive behavior test, or (iv) oil, but no behavior test. Results showed that following lordosis, there was increased c-Fos expression in the medial bed nucleus of the stria terminalis, medial accumbens, medial preoptic nucleus, paraventricular nucleus and medial amygdala. Following a single aggression test, c-Fos was significantly increased only within the medial amygdala. There was no effect of lordosis or aggression on c-Fos expression within the lateral or central ventromedial hypothalamus, suprachiasmatic nucleus or dorsal midbrain central gray. In a second experiment, ovariectomized female hamsters were given (i) repeated aggressive experience, (ii) a single aggression test or (iii) no aggression test. Because some females were not aggressive towards males, they became a separate group post hoc. The number of cells expressing c-Fos was higher in the medial preoptic nucleus and medial amygdala of females given a single aggressive test and in non-aggressive females vs control females. Females given prior aggressive experience showed higher c-Fos expression only in the medial preoptic nucleus. These results demonstrate that increased neural activation in several forebrain nuclei is seen after sexual or aggressive behaviors in female hamsters. However, because the pattern of c-Fos staining in the non-aggressive females was similar to the pattern in aggressive females, this questions previous conclusions regarding the behavioral specificity of these effects and suggests instead that such activation is common to social interactions in general.
这些实验的目的是利用c-Fos免疫细胞化学技术来确定雌性仓鼠大脑中在脊柱前凸和攻击行为期间活跃的区域。对卵巢切除的仓鼠进行如下处理：（i）给予雌二醇和孕酮，并进行脊柱前凸测试；（ii）给予雌二醇和孕酮，但不进行脊柱前凸测试；（iii）给予油剂，并进行攻击行为测试；或（iv）给予油剂，但不进行行为测试。结果显示，在脊柱前凸行为后，终纹床核内侧、伏隔核内侧、视前内侧核、室旁核和杏仁核内侧的c-Fos表达增加。在单次攻击测试后，仅杏仁核内侧的c-Fos显著增加。脊柱前凸或攻击行为对下丘脑外侧或腹内侧中央、视交叉上核或中脑背侧中央灰质中的c-Fos表达没有影响。在第二个实验中，对卵巢切除的雌性仓鼠进行如下处理：（i）重复攻击经历；（ii）单次攻击测试；或（iii）不进行攻击测试。由于一些雌性对雄性没有攻击性，因此在事后它们成为了一个单独的组。在接受单次攻击测试的雌性以及非攻击性雌性的视前内侧核和杏仁核内侧中，表达c-Fos的细胞数量高于对照雌性。有过攻击经历的雌性仅在视前内侧核中显示出较高的c-Fos表达。这些结果表明，雌性仓鼠在性行为或攻击行为后，几个前脑核中的神经激活增加。然而，由于非攻击性雌性中的c-Fos染色模式与攻击性雌性中的模式相似，这对先前关于这些效应的行为特异性的结论提出了质疑，相反表明这种激活在一般社交互动中是常见的。
