Vrensen G F, Duindam H J
Department of Morphology, The Netherlands Ophthalmic Research Institute, Amsterdam.
Ophthalmic Res. 1995;27 Suppl 1:78-85. doi: 10.1159/000267846.
Electron microscopic, freeze fracture and cholesterol cytochemical studies have revealed that the maturation of lens fibers is accompanied by considerable changes in the ultrastructure of their limiting membranes. These changes are in line with biochemical studies on the cholesterol content in the lens and support the physiological notion that upon maturation lens membranes are transformed from normal permeable to nonleaky membranes. In addition it is shown that early cortical changes involved disturbances of lens membranes. As a consequence it can be postulated that, in order to be significant, future studies on the early events leading to cataractous disturbances must be carried out using a combination of histochemical, immunohistochemical and regional biochemical approaches.
电子显微镜、冷冻断裂及胆固醇细胞化学研究表明，晶状体纤维的成熟伴随着其界膜超微结构的显著变化。这些变化与晶状体中胆固醇含量的生化研究结果一致，并支持了这样一种生理学观点，即晶状体膜在成熟时从正常可渗透膜转变为不透膜。此外，研究还表明，早期皮质变化涉及晶状体膜的紊乱。因此可以推测，为了具有重要意义，未来关于导致白内障紊乱的早期事件的研究必须结合组织化学、免疫组织化学和区域生化方法来进行。
