Horsch A K, Hudson K, Day A J
Atherosclerosis. 1977 Apr;26(4):493-504. doi: 10.1016/0021-9150(77)90117-4.
Following the intravenous injection of tracer doses of 3H-fatty acid labelled lecithin into normally fed rabbits, minimal incorporation into serum cholesterol ester over a 6-hour period was observed. The labelled phospholipid was rapidly removed from the serum primarily by R.E. tissues with little uptake by the aorta. The uptake and metabolism of 3H-fatty acid labelled lecithin by explants of normal and cholesterol-fed rabbit aortas was also investigated in vitro for periods up to 8 days. Low uptake and incorporation into cholesterol ester was observed for the normal aortic explants. The labelled phospholid was taken up to a greater extent by explants from cholesterol-fed rabbit aortas and the fatty acid incorporated into both triglyceride and cholesterol ester in the explants. The incorporation of fatty acid into cholesterol ester from phospholipid was associated with hydrolysis of the phospholipid and re-incorporation of the fatty acid into cholesterol ester, rather than by the action of lecithin cholesterol acyl transferase.
给正常喂食的兔子静脉注射微量经³H-脂肪酸标记的卵磷脂后，观察到在6小时内血清胆固醇酯中的掺入量极少。标记的磷脂主要通过网状内皮组织迅速从血清中清除，主动脉摄取很少。还对正常和喂食胆固醇的兔主动脉外植体在体外长达8天的时间内对³H-脂肪酸标记的卵磷脂的摄取和代谢情况进行了研究。正常主动脉外植体对胆固醇酯的摄取和掺入量较低。喂食胆固醇的兔主动脉外植体对标记的磷脂摄取程度更高，脂肪酸掺入外植体中的甘油三酯和胆固醇酯中。脂肪酸从磷脂掺入胆固醇酯的过程与磷脂的水解以及脂肪酸重新掺入胆固醇酯有关，而不是通过卵磷脂胆固醇酰基转移酶的作用。
