Wang J, Brunner C J, Gangopadhyay A, Bird A C, Wolfe L G
Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5519, USA.
Vet Immunol Immunopathol. 1995 Oct;48(3-4):193-207. doi: 10.1016/0165-2427(95)05436-a.
Two murine monoclonal antibodies (MAbs), 1A10 and SB2, generated against a canine mammary carcinoma cell line, were used in a competitive enzyme-linked immunosorbent assay (ELISA) to measure tumor-associated antigens (TAAs) in canine serum samples. Sera were tested from disease-free dogs and from dogs diagnosed with mammary carcinoma, non-mammary carcinoma, sarcoma, benign mammary tumor, benign non-mammary tumor, or non-neoplastic disease. Serum antigen concentrations measured by ELISA were expressed as inhibitory units (IU). The upper limit of normal, defined as the mean plus 2 SD of the TAA concentration in disease-free dogs, was 20 IU with antibody 1A10 and 22 IU with antibody SB2. Compared with disease-free dogs, the frequency of TAA-positive sera was significantly greater (P < 0.05) among dogs with mammary or non-mammary carcinoma when tested with MAbs 1A10 or SB2, and also with sarcoma when tested with MAb SB2. Testing a serum sample with both antibodies rather than just one increased the sensitivity of the competitive ELISA for TAA detection. The presence of TAA in serum might serve as a useful marker for certain types of carcinomas or sarcomas in canine cancer patients.
两种针对犬乳腺癌细胞系产生的鼠单克隆抗体（MAb），即1A10和SB2，用于竞争性酶联免疫吸附测定（ELISA），以检测犬血清样本中的肿瘤相关抗原（TAA）。对无病犬以及被诊断患有乳腺癌、非乳腺癌、肉瘤、良性乳腺肿瘤、良性非乳腺肿瘤或非肿瘤性疾病的犬的血清进行了检测。通过ELISA测量的血清抗原浓度以抑制单位（IU）表示。正常上限定义为无病犬TAA浓度的平均值加2个标准差，使用抗体1A10时为20 IU，使用抗体SB2时为22 IU。与无病犬相比，在用MAb 1A10或SB2检测时，患有乳腺癌或非乳腺癌的犬中TAA阳性血清的频率显著更高（P < 0.05），在用MAb SB2检测肉瘤时也是如此。用两种抗体而非仅一种抗体检测血清样本可提高竞争性ELISA检测TAA的灵敏度。血清中TAA的存在可能作为犬癌症患者某些类型的癌或肉瘤的有用标志物。
