Li S, Woo P T
Department of Zoology, University of Guelph, Ont., Canada.
Vet Immunol Immunopathol. 1995 Oct;48(3-4):343-53. doi: 10.1016/0165-2427(95)05445-c.
An attenuated strain of Cryptobia salmositica was used as a live vaccine to protect rainbow trout, Oncorhynchus mykiss, against cryptobiosis. Fish immunized with 1.0 x 10(4) or 5.5 x 10(4) attenuated C. salmositica per fish were protected 3 weeks after immunization; however, this period was reduced to 2 weeks if fish were vaccinated with 1.0 x 10(5) attenuated parasites per fish. Fish were still protected at 6, 12 and 24 months after vaccination. Complement fixing antibodies in the blood of immunized fish lysed C. salmositica under in vitro conditions. The titre of complement fixing antibodies in vaccinated fish increased rapidly 1-2 weeks post-challenge. In vitro phagocytosis was enhanced by antiserum and by activated macrophages from vaccinated fish. There was also evidence of antibody independent and antibody dependent cell-mediated cytotoxicity in vaccinated fish.
一种减毒的鲑隐鞭虫菌株被用作活疫苗，以保护虹鳟（Oncorhynchus mykiss）免受隐生作用的影响。每尾鱼用1.0×10⁴或5.5×10⁴减毒的鲑隐鞭虫免疫后，在免疫3周后得到保护；然而，如果每尾鱼用1.0×10⁵减毒寄生虫进行疫苗接种，这个时期会缩短到2周。在接种疫苗后的6、12和24个月，鱼仍然受到保护。免疫鱼血液中的补体结合抗体在体外条件下可裂解鲑隐鞭虫。接种疫苗的鱼在攻击后1-2周，补体结合抗体的滴度迅速增加。抗血清和接种疫苗鱼的活化巨噬细胞可增强体外吞噬作用。在接种疫苗的鱼中也有抗体非依赖和抗体依赖的细胞介导细胞毒性的证据。
