Rejman J J, Luther D A, Owens W E, Nickerson S C, Oliver S P
Department of Animal Science, University of Tennessee, Knoxville 37901-1071, USA.
Zentralbl Veterinarmed B. 1995 Oct;42(8):449-58. doi: 10.1111/j.1439-0450.1995.tb00736.x.
This study was designed to determine whether intramammary infusion of recombinant bovine interleukin-1 beta or interleukin-2 had any adverse influence on bovine mammary function during the early non-lactating period. Mammary glands of eight Jersey cows were infused with either 10 micrograms of interleukin-1 beta or 1.0 mg of interleukin-2 following abrupt cessation of milking. Mammary secretions from each gland were collected from cows frequently during early involution and evaluated for changes in secretion composition. Percentage of milk fat and solids-not-fat during the experimental period was variable for all treatments. Percentage of protein increased throughout the study for all treatments, but mammary glands treated with interleukin-1 beta had higher protein percentages. All interleukin-treated mammary glands had lower concentrations of lactose than controls. Mammary secretions from interleukin-1 beta treated glands had higher concentrations of lactoferrin at 7 and 14 days of involution. Similarly, mammary secretions from interleukin-2 treated glands had higher concentrations of serum albumin on day 3 of involution and throughout the remainder of the study. Concentration of citrate and the citrate: lactoferrin molar ratio generally decreased throughout the study for all treatment groups. Concentration of alpha-lactalbumin in mammary secretions decreased throughout the study and was not different between treatment groups. Though significant changes in mammary secretion composition due to the presence of cytokines were observed in this study, resulting in an apparent acceleration of involution, there was no indication that these changes adversely affected normal bovine mammary function during the early non-lactating period.
本研究旨在确定在非泌乳早期向奶牛乳房内注射重组牛白细胞介素-1β或白细胞介素-2是否会对奶牛乳房功能产生任何不利影响。在突然停止挤奶后，向8头泽西奶牛的乳房内注射10微克白细胞介素-1β或1.0毫克白细胞介素-2。在早期 involution 期间频繁从奶牛身上收集每个乳房的分泌物，并评估分泌物成分的变化。所有处理组在实验期间乳脂肪和非脂固形物的百分比均有变化。所有处理组的蛋白质百分比在整个研究过程中均有所增加，但用白细胞介素-1β处理的乳房蛋白质百分比更高。所有用白细胞介素处理的乳房乳糖浓度均低于对照组。在 involution 的第7天和第14天，用白细胞介素-1β处理的乳房分泌物乳铁蛋白浓度较高。同样，在 involution 的第3天及整个研究的其余时间，用白细胞介素-2处理的乳房分泌物血清白蛋白浓度较高。所有处理组的柠檬酸盐浓度和柠檬酸盐:乳铁蛋白摩尔比在整个研究过程中总体上均下降。乳房分泌物中α-乳白蛋白的浓度在整个研究过程中下降，各处理组之间无差异。尽管在本研究中观察到由于细胞因子的存在导致乳房分泌物成分发生显著变化，从而导致 involution 明显加速，但没有迹象表明这些变化在非泌乳早期对正常奶牛乳房功能产生不利影响。
