亲和电泳在抗原 - 免疫吸附剂缔合平衡研究中的应用。

Application of affinity electrophoresis to the study of antigen-immunoadsorbent association equilibrium.

作者信息

Caron M, Faure A, Keros R G, Cornillot P

出版信息

Biochim Biophys Acta. 1977 Apr 25;491(2):558-65. doi: 10.1016/0005-2795(77)90301-4.

Abstract

Affinity electrophoresis is used to study the interaction of complementary biological molecules. Using Scatchard plots, this method gives a partition ratio between immunoadsorbent and soluble antigen or antibody. In the present work, affinity electrophoresis, used to study the interaction between human serum albumin and its insolubilized antibodies, allows a measure of the system's affinity under specific conditions, in particular, the electric field.

摘要

亲和电泳用于研究互补生物分子之间的相互作用。通过使用斯卡查德图，该方法可得出免疫吸附剂与可溶性抗原或抗体之间的分配比。在本研究中，亲和电泳用于研究人血清白蛋白与其固定化抗体之间的相互作用，能够测定特定条件下，尤其是电场条件下该系统的亲和力。